Histone H3.3 Polyclonal antibody

• Featured Product
• KD/KO Validated

Histone H3.3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 13754-1-AP

Synonyms

H3F3A, HistoneH3.3, Histone H3, H3-3B, H3-3A

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Validation Data Gallery

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  WB analysis using 13754-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13754-1-AP (Histone H3.3 antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse kidney using 13754-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13754-1-AP (Histone H3.3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 13754-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13754-1-AP (Histone H3.3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13754-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13754-1-AP (H3F3A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13754-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13754-1-AP (H3F3A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 13754-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13754-1-AP (H3F3A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 13754-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13754-1-AP (Histone H3.3 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 13754-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Histone H3.3 antibody (13754-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, HEK-293 cells, mouse thymus tissue, mouse kidney tissue, mouse lung tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 1 publications below

Product Information

13754-1-AP targets Histone H3.3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Histone H3.3 fusion protein Ag4696
• Full Name: H3 histone, family 3A
• Calculated Molecular Weight: 136 aa, 15 kDa
• Observed Molecular Weight: 15-17 kDa
• GenBank Accession Number: BC038989
• Gene Symbol: Histone H3
• Gene ID (NCBI): 3020
• RRID: AB_2115140
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Histones are the basic nuclear proteins responsible for the nucleosome structure of the chromosomal fiber. Five classes of histone genes have been reported, some of which are expressed only during S phase, while others are replication independent. The latter are referred to as replacement histones and are expressed in quiescent or terminally differentiated cells. [PMID: 8586426] Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. H3F3A is one replacement histone [PMID:16258499].

Publications

Species	Application	Title
human	WB	Int J Mol Sci; ADAM10 Plasma and CSF Levels Are Increased in Mild Alzheimer's Disease.; Authors - Izabela Pereira Vatanabe
human	WB	Biochem Biophys Res Commun; Protective effects of 4-octyl itaconate against inflammatory response in angiotensin II-induced oxidative stress in human primary retinal pigment epithelium.; Authors - Xinyu Fu
rat	IF	Fish Shellfish Immunol; Triclocarban evoked neutrophil extracellular trap formation in common carp (Cyprinus carpio L.) by modulating SIRT3-mediated ROS crosstalk with ERK1/2/p38 signaling; Authors - Siwen Li
human	WB	Nat Commun; Impaired histone inheritance promotes tumor progression; Authors - Congcong Tian
human	WB	Clin Transl Med; LncRNA NEAT1 suppresses cellular senescence in hepatocellular carcinoma via KIF11-dependent repression of CDKN2A; Authors - Danlei Chen ; KD Validated
		J Hepatol; IL-6/STAT3 axis dictates the PNPLA3-mediated susceptibility to non-alcoholic fatty liver disease; Authors - Jiwoon Park