H3F3B Polyclonal antibody

H3F3B Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 10265-1-AP

Synonyms

H3F3A, H3F3, H3-3B, H3-3A, H3.3B

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Validation Data Gallery

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  WB analysis of HEK-293 using 10265-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10265-1-AP (H3F3B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10265-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10265-1-AP (H3F3B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

10265-1-AP targets H3F3B in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: H3F3B fusion protein Ag0326
• Full Name: H3 histone, family 3B (H3.3B)
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC001124
• Gene Symbol: H3F3B
• Gene ID (NCBI): 3021
• RRID: AB_513398
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Histones are the basic nuclear proteins responsible for the nucleosome structure of the chromosomal fiber. Five classes of histone genes have been reported, some of which are expressed only during S phase, while others are replication independent. The latter are referred to as replacement histones and are expressed in quiescent or terminally differentiated cells. H3F3B is one replacement histone [PMID: 8586426]. It constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Thereby it has a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability [PMID: 14718166, 15776021].

Publications

Species	Application	Title
human	WB	Cell Commun Signal; Reciprocal regulation between RACGAP1 and AR contributes to endocrine therapy resistance in prostate cancer; Authors - Jiajia Wang
mouse	WB	Immunobiology; Exploring liquid-liquid phase separation-related diagnostic biomarkers in osteoarthritis based on machine learning algorithms and experiment; Authors - Bo Dong