KDM5C Polyclonal antibody

KDM5C Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 14426-1-AP

Synonyms

Lysine-specific demethylase 5C, Jumonji/ARID domain-containing protein 1C, JARID1C, EC:1.14.11.67, DXS1272E

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Validation Data Gallery

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  WB analysis using 14426-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14426-1-AP (KDM5C antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 14426-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14426-1-AP (KDM5C antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, MCF-7 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

14426-1-AP targets KDM5C in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KDM5C fusion protein Ag5791
• Full Name: lysine (K)-specific demethylase 5C
• Calculated Molecular Weight: 176 kDa
• Observed Molecular Weight: 171-180 kDa
• GenBank Accession Number: BC054499
• Gene Symbol: KDM5C
• Gene ID (NCBI): 8242
• RRID: AB_10837073
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KDM5C belongs to the ARID protein family that harbors conserved DNA-binding motifs and acts as a histone H3 lysine 4 demethylase, suggesting a potential role in epigenetic regulation during development, cell differentiation (PMID:21575681). KDM5C protein is homologous to three other proteins of the human JARID1 family (JARID1A, JARID1B and JARID1C). This family shares several evolutionarily conserved domains, including the catalytic JmjC domain, JmjN domain, Arid/Bright domain, C5HC2 zinc-finger domain and two plant homeodomains (PHD), a motif that binds methyl-lysine residues (PMID:17320160,17320161).

Publications

Species	Application	Title
human	WB,IP	Cancer Res; KDM5C-mediated recruitment of BRD4 to chromatin regulates enhancer activation and BET inhibitor sensitivity; Authors - Yulong Qiang
human	WB	Int J Biol Sci; KDM5B demethylates H3K4 to recruit XRCC1 and promote chemoresistance.; Authors - Wenxia Xu
human	WB,IF	Neurol Genet; Novel Variations in the KDM5C Gene Causing X-Linked Intellectual Disability.; Authors - Po-Ming Wu
mouse	WB	Immunohorizons; Inhibition of H3K27me3 Demethylases Promotes Plasmablast Formation.; Authors - Anna K Kania
human	WB	Nat Metab; Myc-mediated SDHA acetylation triggers epigenetic regulation of gene expression and tumorigenesis.; Authors - Shi-Ting Li