Anticorps Monoclonal anti-HSP60

HSP60 Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

34

CloneNo.

2F10E7

N° de cat : 66041-1-Ig

Synonymes

60 kDa chaperonin, Chaperonin 60, GROEL, Heat shock protein 60, HSP 60, HSP60, HSP65, HSPD1

---

Galerie de données de validation

• 

  WB analysis using 66041-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66041-1-Ig (HSP60 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  IP experiment of mouse liver using 66041-1-Ig

  IP Result of anti-HSP60 (IP:66041-1-Ig, 5ug; Detection:66041-1-Ig 1:2000) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human ovary tumor using 66041-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66041-1-Ig (HSP60 antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 66041-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66041-1-Ig (HSP60 antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 66041-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66041-1-Ig (HSP60 antibody at dilution of 1:500 (under 10x lens).

• 

  IHC staining of human spleen using 66041-1-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen using 66041-1-Ig(HSP60 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 66041-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 66041-1-Ig(HSP60 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 66041-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 66041-1-Ig(HSP60 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 66041-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSP60 antibody (66041-1-Ig, Clone: 2F10E7 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), (CL594-Phalloidin, red).

• 

  IF Staining of Neuro-2a using 66041-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using HSP60 antibody (66041-1-Ig, Clone: 2F10E7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HeLa using 66041-1-Ig

  1X10^6 HeLa cells were stained with 0.2ug HSP60 antibody (66041-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HSC-T6, cellules Jurkat, cellules NIH/3T3, cerveau de lapin, cerveau de porc, cerveau de poulet
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu d'amygdalite humain, tissu de cancer de la peau humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, cellules Neuro-2a
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 23 publications below
• IF: See 14 publications below
• IP: See 2 publications below
• ELISA: See 1 publications below

Informations sur le produit

66041-1-Ig cible HSP60 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, levure, porc, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HSP60 Protéine recombinante Ag7848
• Nom complet: heat shock 60kDa protein 1 (chaperonin)
• Masse moléculaire calculée: 61 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC003030
• Symbole du gène: HSPD1
• Identification du gène (NCBI): 3329
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HSPD1, also known as HSP60, belongs to the chaperonin family and acts as a chaperone to enhance cell survival under physiological stresses. Hsp60 has been shown to be connected with many aspects of cell functions such as protein folding and assembling of polypeptide chains in mitochondria. Recently it has been reported that HSP60 is associated with apoptosis or inhibition of cancer cell growth. (21822415)

Publications

Species	Application	Title
human	WB	Nat Med; The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity.; Authors - Wenzhang Wang
human	WB, IF	Nat Immunol; Kinases Mst1 and Mst2 positively regulate phagocytic induction of reactive oxygen species and bactericidal activity.; Authors - Jing Geng
human	WB	Autophagy; Substitution of PINK1 Gly411 modulates substrate receptivity and turnover; Authors - Fabienne C Fiesel
mouse	WB	J Clin Invest; ATM inhibition enhances cancer immunotherapy by promoting mtDNA leakage/cGAS-STING activation.; Authors - Mengjie Hu
mouse	IF	Autophagy; Increased mitophagy protects cochlear hair cells from aminoglycoside-induced damage.; Authors - Yuhua Zhang
mouse	WB	Autophagy; Mycobacterium bovis induces mitophagy to suppress host xenophagy for its intracellular survival.; Authors - Yinjuan Song

Produits associés

HSP60 Polyclonal antibody

15282-1-AP

HSP70 Polyclonal antibody

10995-1-AP

HSP90 Polyclonal antibody

13171-1-AP

HSP27 Polyclonal antibody

18284-1-AP

IDH1 Polyclonal antibody

12332-1-AP

GRP78/BIP Polyclonal antibody

11587-1-AP