IDH1 Polyclonal antibody

• Featured Product
• KD/KO Validated

IDH1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

56

Reviews (1)

Cat no : 12332-1-AP

Synonyms

IDP, IDH 1, IDH, IDCD, EC:1.1.1.42

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Validation Data Gallery

• 

  WB analysis using 12332-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12332-1-AP (IDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 12332-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 12332-1-AP (IDH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 12332-1-AP

  IP result of anti-IDH1 (IP:12332-1-AP, 4ug; Detection:12332-1-AP 1:500) with HepG2 cells lysate 1880 ug.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 12332-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 12332-1-AP (IDH1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12332-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12332-1-AP (IDH1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 12332-1-AP

  Immunofluorescent analysis of HepG2 cells using 12332-1-AP (IDH1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: HEK-293 cells, rat liver tissue, HeLa cells, HepG2 cells, NIH/3T3 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 50 publications below
• IHC: See 8 publications below
• IF: See 2 publications below
• IP: See 5 publications below
• CoIP: See 2 publications below

Product Information

12332-1-AP targets IDH1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IDH1 fusion protein Ag2986
• Full Name: isocitrate dehydrogenase 1 (NADP+), soluble
• Calculated Molecular Weight: 414 aa, 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC012846
• Gene Symbol: IDH1
• Gene ID (NCBI): 3417
• RRID: AB_2123159
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IDH1, also named as PICD and IDP, belongs to the isocitrate and isopropylmalate dehydrogenases family. It is a common feature of a major subset of primary human brain cancers. It can form a homodimer(PMID:15173171). IDH1 mutation is always heterozygotic and IDH1 functions as a dimer, theoretically there will be 25% each wild type and mutant homo-dimers and 50% hetero-dimers present in the tumor cells(PMID:21079649 ).

Publications

Species	Application	Title
mouse	WB	Nature; α-Ketoglutarate links p53 to cell fate during tumour suppression.; Authors - John P Morris ; KD Validated
human	WB,IHC	Nature; IDH mutation impairs histone demethylation and results in a block to cell differentiation.; Authors - Lu Chao C
human	WB	Nature; Cancer-associated IDH1 mutations produce 2-hydroxyglutarate.; Authors - Dang Lenny L
human	WB	Nature; Quantitative flux analysis reveals folate-dependent NADPH production.; Authors - Jing Fan
human	WB	Cancer Cell; Leukemic IDH1 and IDH2 mutations result in a hypermethylation phenotype, disrupt TET2 function, and impair hematopoietic differentiation.; Authors - Maria E Figueroa
human	WB	Cell Stem Cell; PHB Associates with the HIRA Complex to Control an Epigenetic-Metabolic Circuit in Human ESCs.; Authors - Zhexin Zhu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Umut (Verified Customer) (07-25-2023)

The antibody worked very well for Western Blot in a standard application with an average dilution and exposure.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: U87MG

IDH1 Antibody Western Blot validation (1:1000 dilution) in U87MG (Cat no:12332-1-AP)

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