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IDH1 Polyclonal antibody
IDH1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 12332-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, rat liver tissue, HeLa cells, HepG2 cells, NIH/3T3 cells |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 7 publications below |
WB | See 48 publications below |
IHC | See 8 publications below |
IF | See 2 publications below |
IP | See 5 publications below |
CoIP | See 2 publications below |
Product Information
12332-1-AP targets IDH1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, sheep |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | IDH1 fusion protein Ag2986 |
Full Name | isocitrate dehydrogenase 1 (NADP+), soluble |
Calculated Molecular Weight | 414 aa, 47 kDa |
Observed Molecular Weight | 47 kDa |
GenBank Accession Number | BC012846 |
Gene Symbol | IDH1 |
Gene ID (NCBI) | 3417 |
RRID | AB_2123159 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IDH1, also named as PICD and IDP, belongs to the isocitrate and isopropylmalate dehydrogenases family. It is a common feature of a major subset of primary human brain cancers. It can form a homodimer(PMID:15173171). IDH1 mutation is always heterozygotic and IDH1 functions as a dimer, theoretically there will be 25% each wild type and mutant homo-dimers and 50% hetero-dimers present in the tumor cells(PMID:21079649 ).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for IDH1 antibody 12332-1-AP | Download protocol |
IHC protocol for IDH1 antibody 12332-1-AP | Download protocol |
IF protocol for IDH1 antibody 12332-1-AP | Download protocol |
IP protocol for IDH1 antibody 12332-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nature IDH mutation impairs histone demethylation and results in a block to cell differentiation. | ||
Cancer Cell Leukemic IDH1 and IDH2 mutations result in a hypermethylation phenotype, disrupt TET2 function, and impair hematopoietic differentiation. | ||
Cell Stem Cell PHB Associates with the HIRA Complex to Control an Epigenetic-Metabolic Circuit in Human ESCs. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Umut (Verified Customer) (07-25-2023) | The antibody worked very well for Western Blot in a standard application with an average dilution and exposure.
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