GRP78/BIP Polyclonal antibody

• Featured Product
• KD/KO Validated

GRP78/BIP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

625

Reviews (7)

Cat no : 11587-1-AP

Synonyms

BIP, GRP78, HSPA5, GRP 78, Endoplasmic reticulum chaperone BiP

---

Validation Data Gallery

• 

  WB analysis using 11587-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11587-1-AP (GRP78/BIP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11587-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11587-1-AP (GRP78/BIP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11587-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11587-1-AP (GRP78/BIP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11587-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 11587-1-AP (GRP78/BIP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 11587-1-AP

  IP result of anti-GRP78/BIP (IP:11587-1-AP, 3ug; Detection:11587-1-AP 1:1000) with MCF-7 cells lysate 3250ug.

• 

  IHC staining of human pancreas cancer using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of rat brain using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human breast cancer using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11587-1-AP (GRP78/BIP antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse heart using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11587-1-AP (GRP78/BIP antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse kidney using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 11587-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GRP78/BIP antibody (11587-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 11587-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GRP78/BIP antibody (11587-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 11587-1-AP

  Immunofluorescent analysis of HepG2 cells using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  FC experiment of HeLa using 11587-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human GRP78/BIP (11587-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive WB detected in: BxPC-3 cells, Caco-2 cells, HEK-293 cells, HeLa cells, HepG2 cells, mouse brain tissue, mouse liver tissue, rat brain tissue, rat liver tissue, HT-29 cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human pancreas cancer tissue, human breast cancer tissue, mouse kidney tissue, rat brain tissue, mouse heart tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 16 publications below
• WB: See 562 publications below
• IHC: See 79 publications below
• IF: See 89 publications below
• IP: See 12 publications below
• CoIP: See 5 publications below

Product Information

11587-1-AP targets GRP78/BIP in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, rabbit, monkey, chicken, fish, carp, medaka embryos, deer
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GRP78/BIP fusion protein Ag2188
• Full Name: heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
• Calculated Molecular Weight: 78 kDa
• Observed Molecular Weight: 70-78 kDa
• GenBank Accession Number: BC020235
• Gene Symbol: GRP78/BIP
• Gene ID (NCBI): 3309
• RRID: AB_2119855
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth. 11587-1-AP antibody recognizes one or two bands with MW 70-78 kDa for the signal peptide of GRP78 protein can be finally cleaved.

Publications

Species	Application	Title
	WB	Science; Translation from the 5' untranslated region shapes the integrated stress response.; Authors - Shelley R Starck
mouse	WB	Cell Metab; Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.; Authors - Xiao Wang
mouse	WB	Cell Metab; TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.; Authors - Dong Huang
mouse	WB	Crit Care; Recombinant ACE2 protein protects against acute lung injury induced by SARS-CoV-2 spike RBD protein.; Authors - Lingbing Zhang
human,rat	WB	Acta Pharm Sin B; SMYD3-PARP16 axis accelerates unfolded protein response and mediates neointima formation.; Authors - Fen Long
human,mouse	WB	J Pineal Res; Melatonin alleviates PM2.5 -induced glucose metabolism disorder and lipidome alteration by regulating endoplasmic reticulum stress; Authors - Zhou Du

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

PK (Verified Customer) (03-22-2022)

• Applications: Western Blot
• Cell Tissue Type: FIBROBLAST

GRP78/BIP Antibody Western Blot validation ( dilution) in FIBROBLAST (Cat no:11587-1-AP)

FH

Yeawon (Verified Customer) (03-17-2022)

Works very good in mouse kidney tissue.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse kidney tissue

FH

Emma (Verified Customer) (03-15-2022)

Works well by WB in LNCaP and CWR22Rv1 cells @ 1:1000. A single band observed.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Prostate

FH

P (Verified Customer) (05-27-2021)

Excellent.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: H9C2

GRP78/BIP Antibody Western Blot validation (1:2000 dilution) in H9C2 (Cat no:11587-1-AP)

FH

Kevin (Verified Customer) (01-24-2019)

Works pretty good in HepG2 cells. One single band was observed.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HepG2

FH

Sylvain (Verified Customer) (12-03-2018)

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: colon cancer cell lines

FH

Natalie (Verified Customer) (11-20-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Rat Dorsal Root Ganglia

GRP78/BIP Antibody Western Blot, validation (1:5000 dilution) in Rat Dorsal Root Ganglia (Cat no:11587-1-AP)

Related Products

Calnexin Polyclonal antibody

10427-2-AP

ATF6 Polyclonal antibody

24169-1-AP

XBP1S/XBP1U Polyclonal antibody

24168-1-AP

ERp57/ERp60 Polyclonal antibody

15967-1-AP

calreticulin Polyclonal antibody

10292-1-AP

GRP94 Monoclonal antibody

60012-1-Ig