Anticorps Polyclonal de lapin anti-HERP

• Phare
• Validé par KD/KO

HERP Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 10813-1-AP

Synonymes

HERP, HERPUD1, KIAA0025, Mif1, SUP

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Galerie de données de validation

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  WB analysis using 10813-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10813-1-AP (HERP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 10813-1-AP

  Tunicamycin treated HepG2 cells were subjected to SDS PAGE followed by western blot with 10813-1-AP (HERP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 10813-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10813-1-AP (HERP antibody) at dilution of 1:100 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules BxPC-3, cellules HeLa, cellules HepG2 traitées à la tunicamycine, cellules LNCaP, cellules U2OS
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

10813-1-AP cible HERP dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HERP Protéine recombinante Ag1084
• Nom complet: homocysteine-inducible, endoplasmic reticulum stress-inducible, ubiquitin-like domain member 1
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: BC008320
• Symbole du gène: HERPUD1
• Identification du gène (NCBI): 9709
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein (HERPUD1), also called HERP or MIF1, is component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. HERP is strongly induced by ER stress but rapidly degraded by proteasome. HERP interacts with members of the ubiquilin family, which function as a shuttle factor to deliver ubiquitinated substrates to the proteasome for degradation. HERP is present in activated microglia in senile plaques in the brain of patients with Alzheimer disease, and there is evidence suggesting HERP increase nigro-striatal dopaminergic activity and is able to improve symptoms in patients with Parkinson's disease, levodopa-related dyskinesias and Tardive dyskinesia. MW of HERP is 54 kDa (PMID: 23620059).

Publications

Species	Application	Title
human	WB	Oncogene; Small extracellular vesicles containing miR-30a-3p attenuate the migration and invasion of hepatocellular carcinoma by targeting SNAP23 gene.; Authors - Chengdong Liu
	WB	J Alzheimers Dis; The Herp protein pathway is not involved in the pro-amyloidogenic effect of hyperhomocysteinemia.; Authors - Zhuo Jia-Min JM
	IF	Biol Reprod; The unfolded protein response contributes to preimplantation mouse embryo death in the DDK syndrome.; Authors - Hao Lanping L
	IHC	PLoS One; C/EBP-beta regulates endoplasmic reticulum stress-triggered cell death in mouse and human models.; Authors - Meir Ofir O
	WB	Exp Cell Res; ER-localized protein-Herpud1 is a new mediator of IL-4-induced macrophage polarization and migration.; Authors - Yiman Li ; KD Validated
human	WB	Mol Cell Biochem; Phe27Cys polymorphism of the human delta opioid receptor predisposes cells to compromised calcium signaling.; Authors - Tuusa Jussi T JT

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