CETP Polyclonal antibody

CETP Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 13459-1-AP

Synonyms

CETP, HDLCQ10, Lipid transfer protein I

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Validation Data Gallery

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  WB analysis of human brain using 13459-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13459-1-AP (CETP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 13459-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CETP antibody (13459-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: human brain tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

13459-1-AP targets CETP in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CETP fusion protein Ag3923
• Full Name: cholesteryl ester transfer protein, plasma
• Calculated Molecular Weight: 493 aa, 55 kDa
• Observed Molecular Weight: 50-70, 100 kDa
• GenBank Accession Number: BC025739
• Gene Symbol: CETP
• Gene ID (NCBI): 1071
• RRID: AB_2082373
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cholesteryl ester transfer protein (CETP) is a plasma glycoprotein which transfers cholesteryl esters, triglycerides, and phospholipids between lipoproteins. In plasma, CETP is mainly associated with HDL particles and catalyzes the transfer of cholesteryl esters from HDL to apolipoprotein (apo) B-containing plasma lipoproteins. Low plasma concentrations of high-density lipoprotein-cholesterol (HDL-C) have long been recognized as a major, independent cardiovascular disease (CVD) risk factor. It demonstrated that altered level of plasma CETP was associated with the increased risk of CVD (PMID: 17991755, PMID: 23477743). CETP can be detected a 70 kDa isoform by glycosylation (PMID: 3281933,PMID: 22056562). It also can be detected 100 kDa dimer (PMID: 7744792).

Publications

Species	Application	Title
human	WB, IF	J Biol Chem; Regulation of hepatic cholesteryl ester transfer protein expression and reverse cholesterol transport by inhibition of DNA topoisomerase II.; Authors - Mengyang Liu
human	IHC	J Transl Med; Characterization of the proteome of stable and unstable carotid atherosclerotic plaques using data-independent acquisition mass spectrometry; Authors - Zhichao Lai