NR4A1 Polyclonal antibody

• Featured Product
• KD/KO Validated

NR4A1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

33

Cat no : 12235-1-AP

Synonyms

NGFIB, NAK1, NAK 1, N10, HMR

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Validation Data Gallery

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  WB analysis using 12235-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12235-1-AP (NR4A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12235-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12235-1-AP (NR4A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12235-1-AP

  IP result of anti-NR4A1 (IP:12235-1-AP, 4ug; Detection:12235-1-AP 1:1000) with HeLa cells lysate 1720 ug.

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  IHC staining of mouse colon using 12235-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12235-1-AP (NR4A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 12235-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12235-1-AP (NR4A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 12235-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12235-1-AP (NR4A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 12235-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12235-1-AP (NR4A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-3 using 12235-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 12235-1-AP (NR4A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, COLO 320 cells, L02 cells, mouse liver tissue, rat liver tissue, PC-3 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse colon tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 26 publications below
• IHC: See 2 publications below
• IF: See 10 publications below
• IP: See 2 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 3 publications below

Product Information

12235-1-AP targets NR4A1 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NR4A1 fusion protein Ag2875
• Full Name: nuclear receptor subfamily 4, group A, member 1
• Calculated Molecular Weight: 598 aa, 64 kDa
• Observed Molecular Weight: 64-70 kDa
• GenBank Accession Number: BC016147
• Gene Symbol: NR4A1
• Gene ID (NCBI): 3164
• RRID: AB_10644125
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NR4A1 is an orphan nuclear receptor that act concomitantly with NURR1 in regulating the expression of delay-early genes during liver regeneration. Also NR4A1 has linked not only to cell apoptosis but also proliferation in response to growth factor. It plays a role in caspase-independent cell death by activation of the ERK pathway and increased activity of MEF2 transcription factors. NR4A1 exists some isoforms with MW 65 and 34 kDa.

Publications

Species	Application	Title
mouse	IF	Aging (Albany NY); A systematic dissection of human primary osteoblasts in vivo at single-cell resolution.; Authors - Yun Gong
rat	WB	Sci Rep; Nur77 exacerbates PC12 cellular injury in vitro by aggravating mitochondrial impairment and endoplasmic reticulum stress.; Authors - Huimin Gao ; KD Validated
mouse	WB,IF	Sci Rep; NR4A1 Knockdown Suppresses Seizure Activity by Regulating Surface Expression of NR2B.; Authors - Yanke Zhang ; KD Validated
mouse	WB, IF	Mol Neurobiol; Contra-directional Coupling of Nur77 and Nurr1 in Neurodegeneration: A Novel Mechanism for Memantine-Induced Anti-inflammation and Anti-mitochondrial Impairment.; Authors - Xiaobo Wei
mouse	WB,IP	Mol Neurobiol; HPGD: An Intermediate Player in Microglial Polarization and Multiple Sclerosis Regulated by Nr4a1; Authors - Mengyang Sun
human,mouse	WB	Bioorg Chem; Structure-based design and synthesis of anti-fibrotic compounds derived from para-positioned 3,4,5-trisubstituted benzene; Authors - Wenbin Hong