HRD1/SYVN1 Polyclonal antibody

• Featured Product
• KD/KO Validated

HRD1/SYVN1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, IP-MS, ELISA

Conjugate

Unconjugated

Publications

60

Cat no : 13473-1-AP

Synonyms

SYVN1, Synovial apoptosis inhibitor 1, KIAA1810, HRD1, EC:2.3.2.27

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Validation Data Gallery

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  WB analysis using 13473-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13473-1-AP (SYVN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13473-1-AP

  WB result of HRD1/SYVN1 antibody (13473-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HRD1/SYVN1 transfected HeLa cells.

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  WB analysis of mouse stomach using 13473-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 13473-1-AP (SYVN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 13473-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 13473-1-AP (SYVN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 13473-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 13473-1-AP (SYVN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-453s using 13473-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 13473-1-AP (SYVN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat stomach using 13473-1-AP

  rat stomach tissue were subjected to SDS PAGE followed by western blot with 13473-1-AP (SYVN1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 13473-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 13473-1-AP (SYVN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13473-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13473-1-AP (SYVN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13473-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13473-1-AP (SYVN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 13473-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13473-1-AP (SYVN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 13473-1-AP

  IP result of anti-HRD1/SYVN1 (IP:13473-1-AP, 4ug; Detection:13473-1-AP 1:1000) with mouse kidney tissue lysate 7200ug.

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  IHC staining of human kidney using 13473-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13473-1-AP (SYVN1 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 13473-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13473-1-AP (SYVN1 antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 13473-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HRD1/SYVN1 antibody (13473-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, MDA-MB-453s cells, mouse kidney tissue, mouse ovary tissue, mouse pancreas tissue, mouse stomach tissue, NIH/3T3 cells, Raji cells, rat stomach tissue
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 14 publications below
• WB: See 57 publications below
• IHC: See 3 publications below
• IF: See 7 publications below
• IP: See 6 publications below
• CoIP: See 5 publications below

Product Information

13473-1-AP targets HRD1/SYVN1 in WB, IHC, IF/ICC, IP, CoIP, IP-MS, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HRD1/SYVN1 fusion protein Ag4267
• Full Name: synovial apoptosis inhibitor 1, synoviolin
• Calculated Molecular Weight: 617 aa, 68 kDa
• Observed Molecular Weight: 68-76 kDa
• GenBank Accession Number: BC030530
• Gene Symbol: HRD1
• Gene ID (NCBI): 84447
• RRID: AB_2287023
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HRD1is also named as SYVN1(Synovial apoptosis inhibitor 1) , KIAA1810. It acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Two distinct binding sites mediate Hrd1 dimerization or oligomerization, one located within the transmembrane region and another within the cytosolic domain. (PMID:19864457).Western blot analysis detected abundant HRD1 expression in liver and kidney. Mouse liver and spleen expressed Hrd1 as an 85-kD protein(PMID:12646171).

Publications

Species	Application	Title
mouse	WB	Cell Metab; AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.; Authors - Hui Luo
human	IP,WB	J Clin Invest; Hypomorphic variants of SEL1L-HRD1 ER-associated degradation are associated with neurodevelopmental disorders; Authors - Huilun Helen Wang
human	WB,IF,IP,IP-MS	Mol Cancer; CircNR3C2 promotes HRD1-mediated tumor-suppressive effect via sponging miR-513a-3p in triple-negative breast cancer.; Authors - Ya Fan
human	WB	Mol Cell; Redundant and Antagonistic Roles of XTP3B and OS9 in Decoding Glycan and Non-glycan Degrons in ER-Associated Degradation.; Authors - Annemieke T van der Goot ; KD Validated
		Mol Cell; Cells Deploy a Two-Pronged Strategy to Rectify Misfolded Proinsulin Aggregates.; Authors - Corey N Cunningham
human	WB	Nat Commun; PGRMC1 acts as a size-selective cargo receptor to drive ER-phagic clearance of mutant prohormones.; Authors - Yu-Jie Chen