• Featured Product
  • KD/KO Validated

HRD1/SYVN1 Polyclonal antibody

HRD1/SYVN1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 13473-1-AP


HRD1, HRD1/SYVN1, KIAA1810, Synovial apoptosis inhibitor 1, SYVN1

Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, NIH/3T3 cells, Raji cells, mouse pancreas tissue, MDA-MB-453s cells, mouse ovary tissue, mouse kidney tissue, mouse stomach tissue, rat stomach tissue, HeLa cells
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13473-1-AP targets HRD1/SYVN1 in WB, IP, IF, IHC, CoIP, IP-MS, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HRD1/SYVN1 fusion protein Ag4267
Full Name synovial apoptosis inhibitor 1, synoviolin
Calculated Molecular Weight 617 aa, 68 kDa
Observed Molecular Weight 68-76 kDa
GenBank Accession NumberBC030530
Gene Symbol SYVN1
Gene ID (NCBI) 84447
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HRD1is also named as SYVN1(Synovial apoptosis inhibitor 1) , KIAA1810. It acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Two distinct binding sites mediate Hrd1 dimerization or oligomerization, one located within the transmembrane region and another within the cytosolic domain. (PMID:19864457).Western blot analysis detected abundant HRD1 expression in liver and kidney. Mouse liver and spleen expressed Hrd1 as an 85-kD protein(PMID:12646171).


Product Specific Protocols
WB protocol for HRD1/SYVN1 antibody 13473-1-APDownload protocol
IHC protocol for HRD1/SYVN1 antibody 13473-1-APDownload protocol
IF protocol for HRD1/SYVN1 antibody 13473-1-APDownload protocol
IP protocol for HRD1/SYVN1 antibody 13473-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Metab

AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.

Authors - Hui Luo

J Clin Invest

Hypomorphic variants of SEL1L-HRD1 ER-associated degradation are associated with neurodevelopmental disorders

Authors - Huilun Helen Wang

Mol Cancer

CircNR3C2 promotes HRD1-mediated tumor-suppressive effect via sponging miR-513a-3p in triple-negative breast cancer.

Authors - Ya Fan

Mol Cell

Redundant and Antagonistic Roles of XTP3B and OS9 in Decoding Glycan and Non-glycan Degrons in ER-Associated Degradation.

Authors - Annemieke T van der Goot
  • KD Validated

Mol Cell

Cells Deploy a Two-Pronged Strategy to Rectify Misfolded Proinsulin Aggregates.

Authors - Corey N Cunningham

Nat Commun

PGRMC1 acts as a size-selective cargo receptor to drive ER-phagic clearance of mutant prohormones.

Authors - Yu-Jie Chen