Anticorps Polyclonal de lapin anti-GR repeat

• Phare
• Validé par KD/KO

GR repeat Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IF, IHC, ELISA, Dot Blot

Conjugaison

Non conjugué

Publications

25

N° de cat : 23978-1-AP

Synonymes

C9RAN, GGGCC, GR repeat, GRGRGRGRG, GRGRGRGRGR, Poly-GR

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Galerie de données de validation

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  WB analysis of Recombinant protein using 23978-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 23978-1-AP (GR repeat Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: Protéine recombinante

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 7 publications below
• IF: See 8 publications below

Informations sur le produit

23978-1-AP cible GR repeat dans les applications de WB, IF, IHC, ELISA, Dot Blot et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Drosophile, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: GR repeat
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The C9orf72 "GGGGCC" repeat sequence codes five repeat peptide "GA repeat; GAGAGAGAGA", "GP repeat; GPGPGPGPG", "GR repeat; GRGRGRGRG", "AP repeat; APAPAPAPA" and "PR repeat; PRPRPRPRP". It was described previously that aggregated forms of poly-GA and poly-GP proteins do not enter the separation gel (PMID: 26374446). This antibody is used to detect the GR repeated sequence. The recombinant proteins GST-SNRPD1 and 6*His tag- SNRPD1 which contains the sequence "GRGRGRGRGRGRGRGRGR" were used to detect this antibody. Both proteins can be recognized by the antibody 23978-1-AP.

Publications

Species	Application	Title
	WB	Nature; The C9orf72 repeat expansion disrupts nucleocytoplasmic transport.; Authors - Ke Zhang
Drosophila	WB	Science; C9orf72 repeat expansions cause neurodegeneration in Drosophila through arginine-rich proteins.; Authors - Sarah Mizielinska
human	IF	Cell; Stress Granule Assembly Disrupts Nucleocytoplasmic Transport.; Authors - Ke Zhang ; KD Validated
human	IHC	Nat Neurosci; RPS25 is required for efficient RAN translation of C9orf72 and other neurodegenerative disease-associated nucleotide repeats.; Authors - Shizuka B Yamada
human		Nat Commun; Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis; Authors - Ji Geng
zebrafish	Dot Blot	Acta Neuropathol; A zebrafish model for C9orf72 ALS reveals RNA toxicity as a pathogenic mechanism.; Authors - Bart Swinnen

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