Anticorps Polyclonal de lapin anti-CD42b

CD42b Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, souris et plus (1)

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

27

N° de cat : 12860-1-AP

Synonymes

BSS, CD42b, CD42b alpha, GP1B, GP1BA

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Galerie de données de validation

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  WB analysis of human peripheral blood platelets using 12860-1-AP

  Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 12860-1-AP (CD42b antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig peripheral blood leukocyte using 12860-1-AP

  pig peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 12860-1-AP (CD42b antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human blood using 12860-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 12860-1-AP (CD42b antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human spleen using 12860-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 12860-1-AP (CD42b antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human spleen using 12860-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 12860-1-AP (CD42b antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse spleen using 12860-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 12860-1-AP (CD42b antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 12860-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 12860-1-AP (CD42b antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 12860-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 12860-1-AP (CD42b antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 12860-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 12860-1-AP (CD42b antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: plaquettes de sang périphérique humain, leucocytes de sang périphérique de porc, sang humain, Sanguin humain
• Résultats positifs en IHC: tissu splénique humain, tissu splénique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 13 publications below
• IHC: See 11 publications below
• IF: See 1 publications below

Informations sur le produit

12860-1-AP cible CD42b dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, porc, souris

• Réactivité: Humain, porc, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD42b Protéine recombinante Ag3893
• Nom complet: glycoprotein Ib (platelet), alpha polypeptide
• Masse moléculaire calculée: 626 aa, 69 kDa
• Poids moléculaire observé: 145 kDa
• Numéro d’acquisition GenBank: BC027955
• Symbole du gène: CD42b
• Identification du gène (NCBI): 2811
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GP1BA, also named as CD42b , is a 145-kDa surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium. It has a heterodimer form which composed of GP1BA and GP1BB.

Publications

Species	Application	Title
human	WB	Adv Healthc Mater; Platelet Membrane-Fused Circulating Extracellular Vesicles Protect the Heart from Ischemia/Reperfusion Injury; Authors - Jizong Jiang
rat	WB	Theranostics; A blood circulation-prolonging peptide anchored biomimetic phage-platelet hybrid nanoparticle system for prolonged blood circulation and optimized anti-bacterial performance.; Authors - Peipei Jin
human	WB	Int J Biol Macromol; Platelet-membrane camouflaged cerium nanoparticle-embedded gelatin methacryloyl hydrogel for accelerated diabetic wound healing; Authors - Hang Dong
mouse	WB	ACS Biomater Sci Eng; Biomimetic Platform Based on Mesoporous Platinum for Multisynergistic Cancer Therapy.; Authors - Gaoqian Zhao
mouse	IHC	Biochem Pharmacol; GSK669, a NOD2 receptor antagonist, inhibits thrombosis and oxidative stress via targeting platelet GPVI.; Authors - Guanxing Pan
mouse	WB	Drug Deliv; Platelets are highly efficient and efficacious carriers for tumor-targeted nano-drug delivery.; Authors - Qi-Rui Li

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