VWF, VWFpp Polyclonal antibody
VWF, VWFpp Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IP, ELISA
Conjugate
Unconjugated
103
Cat no : 11778-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HUVEC cells, Jurkat cells, human placenta tissue, mouse spleen tissue, rat spleen tissue |
Positive IP detected in | mouse spleen tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 31 publications below |
Product Information
11778-1-AP targets VWF, VWFpp in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig, monkey |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | VWF, VWFpp fusion protein Ag2386 |
Full Name | von Willebrand factor |
Calculated Molecular Weight | 309 kDa |
Observed Molecular Weight | 309-320 kDa, 75-100 kDa |
GenBank Accession Number | . |
Gene Symbol | VWF |
Gene ID (NCBI) | 7450 |
RRID | AB_10642840 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Von Willebrand factor (vWF) is a large multimeric glycoprotein that is crucial to the hemostasis process. It supports platelet adhesion and carries factor VIII (FVIII). Deficiency of vWF results in von Willebrand disease (vWD), a common inherited bleeding disorder. The pre-pro-vWF is comprised of 2,813 amino acids (aa) which encompass a 22-aa signal peptide, a 741-aa large propeptide (vWF propeptide, vWFpp, also called von Willebrand antigen 2) and 2,050 aa making up the mature vWF. The von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules. This antibody raised against the N-terminal region of pre-pro-vWF recognizes von Willebrand antigen 2 (75-100 kDa) and pro-vWF (309-320 kDa). (PMID: 9759493; 12067899; 22452980; 8874191)
Protocols
Product Specific Protocols | |
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WB protocol for VWF, VWFpp antibody 11778-1-AP | Download protocol |
IP protocol for VWF, VWFpp antibody 11778-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Thromb Haemost Knock-in mice bearing constitutively active αIIb(R990W) mutation develop macrothrombocytopenia with severe platelet dysfunction. | ||
Circulation Hyperglycemia inhibits cardiac stem cell-mediated cardiac repair and angiogenic capacity. | ||
Circulation Intramyocardial peptide nanofiber injection improves postinfarction ventricular remodeling and efficacy of bone marrow cell therapy in pigs. | ||
Acta Pharm Sin B The substitution of SERCA2 redox cysteine 674 promotes pulmonary vascular remodeling by activating IRE1α/XBP1s pathway. | ||
Adv Healthc Mater A ROS-Responsive Liposomal Composite Hydrogel Integrating Improved Mitochondrial Function and Pro-Angiogenesis for Efficient Treatment of Myocardial Infarction. | ||
ACS Appl Mater Interfaces Aligned Graphene Mesh-Supported Double Network Natural Hydrogel Conduit Loaded with Netrin-1 for Peripheral Nerve Regeneration. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Kim-Anh (Verified Customer) (09-03-2019) | Good primary antibody, I used for IF test on HUVECs
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