Anticorps Polyclonal de lapin anti-GATA4-Specific

• Phare
• Validé par KD/KO

GATA4-Specific Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IF, IHC, CoIP, ChiP, ELISA

Conjugaison

Non conjugué

Publications

35

N° de cat : 19530-1-AP

Synonymes

GATA binding factor 4, GATA binding protein 4, GATA4, GATA4-Specific, Transcription factor GATA 4

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Galerie de données de validation

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  WB analysis using 19530-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19530-1-AP (GATA4-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 19530-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19530-1-AP (GATA4-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 19530-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 19530-1-AP (GATA4-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 19530-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 19530-1-AP (GATA4-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules NIH/3T3, cellules PC-3, tissu cardiaque de rat, tissu cardiaque de souris, tissu hépatique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 24 publications below
• IHC: See 4 publications below
• IF: See 11 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 2 publications below

Informations sur le produit

19530-1-AP cible GATA4-Specific dans les applications de WB, IP, IF, IHC, CoIP, ChiP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: GATA binding protein 4
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: NM_002052
• Symbole du gène: GATA4
• Identification du gène (NCBI): 2626
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

What is the molecular weight of GATA4? Is GATA4 post-translationally modified?

The molecular weight of GATA4 is 48 kDa. GATA4 can be phosphorylated, which regulates its activity (PMID: 10788492).

 What is the subcellular localization of GATA4?

GATA4 localizes to the nucleus. The nuclear localization motif is present in the C-terminal part of GATA4 (PMID: 9079680).

 What is the tissue expression pattern of GATA4?

GATA4 was initially identified as a cardiac transcription factor important for the development of the heart and as one of the earliest transcription factors expressed in developing cardiac cells. However, GATA4 is expressed in numerous organs such as the liver, lung, gonads, and small intestine (PMID: 8455608).

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
mouse	WB,IHC,IF,IP	Clin Transl Med; Bmi-1-RING1B prevents GATA4-dependent senescence-associated pathological cardiac hypertrophy by promoting autophagic degradation of GATA4.; Authors - Haiyun Chen
human	IF	Elife; Induction of Sertoli-like cells from human fibroblasts by NR5A1 and GATA4.; Authors - Jianlin Liang
human	WB	Cardiovasc Res; Circular RNA circRNA_000203 aggravates cardiac hypertrophy via suppressing miR26b-5p and miR-140-3p binding to Gata4.; Authors - Hui Li
mouse	WB	J Bone Miner Res; Bmi-1 Overexpression Improves Sarcopenia Induced by 1,25(OH)2 D3 Deficiency and Downregulates GATA4-Dependent Rela Transcription; Authors - Qiuyi Wang
mouse	WB	Cell Prolif; Carbon monoxide alleviates senescence in diabetic nephropathy by improving autophagy.; Authors - Li Chen

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