MEF2C Polyclonal antibody

• Featured Product
• KD/KO Validated

MEF2C Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, chIP, ELISA

Conjugate

Unconjugated

Publications

45

Cat no : 10056-1-AP

Synonyms

Myocyte-specific enhancer factor 2C, myocyte enhancer factor 2C

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 10056-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10056-1-AP (MEF2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 10056-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10056-1-AP (MEF2C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 10056-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 10056-1-AP (MEF2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 10056-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10056-1-AP (MEF2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 10056-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 10056-1-AP (MEF2C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 10056-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 10056-1-AP (MEF2C Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SH-SY5Y using 10056-1-AP

  IP result of anti-MEF2C (IP:10056-1-AP, 3ug; Detection:10056-1-AP 1:500) with SH-SY5Y cells lysate 2000ug.

• 

  IP experiment of SH-SY5Y using 10056-1-AP

  IP result of anti-MEF2C (IP:10056-1-AP, 3ug; Detection:10056-1-AP 1:500) with SH-SY5Y cells lysate 2000ug.

• 

  IP experiment using 10056-1-AP

  IP result of anti-MEF2C (10056-1-AP for IP and Detection) with SHSY5Y cell.

• 

  IHC staining of human lymphoma using 10056-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10056-1-AP (MEF2C antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 10056-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MEF2C antibody (10056-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 10056-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human MEF2C (10056-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, mouse colon tissue, mouse brain tissue, mouse heart tissue
• Positive IP detected in: SH-SY5Y cells
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 25 publications below
• IHC: See 9 publications below
• IF: See 16 publications below
• ChIP: See 2 publications below

Product Information

10056-1-AP targets MEF2C in WB, IHC, IF/ICC, FC (Intra), IP, chIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig, bovine, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MEF2C fusion protein Ag0020
• Full Name: myocyte enhancer factor 2C
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 45-70 kDa
• GenBank Accession Number: BC156603
• Gene Symbol: MEF2C
• Gene ID (NCBI): 4208
• RRID: AB_513447
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MEF2C belongs to the MEF2 family. It is a transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. MEF2C controls cardiac morphogenesis and myogenesis, and is also involved in vascular development[PMID: 20221419]. It plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission[PMID:18599438]. It is crucial for normal neuronal development, distribution, and electrical activity in the neocortex and is necessary for proper development of megakaryocytes and platelets and for bone marrow B lymphopoiesis[PMID: 21666133]. This protein is required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B cells. It may also be involved in neurogenesis and in the development of cortical architecture. MEF2C exists some isoforms with MV 50-52 kDa, 47 kDa, and 45 kDa, but modified MEF2C is about 55-66 kDa.

Publications

Species	Application	Title
human	IF	Nat Biotechnol; Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells.; Authors - Drukker Micha M
human,mouse	IF	Cell Res; Sequential progenitor states mark the generation of pancreatic endocrine lineages in mice and humans.; Authors - Xin-Xin Yu
human	WB	Nat Neurosci; Stimulus-specific combinatorial functionality of neuronal c-fos enhancers.; Authors - Jae-Yeol Joo
mouse	IHC	Nat Neurosci; Tbr1 haploinsufficiency impairs amygdalar axonal projections and results in cognitive abnormality.; Authors - Tzyy-Nan Huang
mouse	WB	Nat Commun; NitroSynapsin therapy for a mouse MEF2C haploinsufficiency model of human autism.; Authors - Shichun Tu
mouse	IHC	Genes Dev; Gsx2 controls region-specific activation of neural stem cells and injury-induced neurogenesis in the adult subventricular zone.; Authors - López-Juárez Alejandro A

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