SRF Polyclonal antibody

• Featured Product
• KD/KO Validated

SRF Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

23

Reviews (1)

Cat no : 16821-1-AP

Synonyms

Serum response factor, MCM1

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Validation Data Gallery

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  WB analysis of MCF-7 using 16821-1-AP

  WB result of SRF antibody (16821-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRF transfected MCF-7 cells.

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  WB analysis using 16821-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16821-1-AP (SRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16821-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16821-1-AP (SRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 16821-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16821-1-AP (SRF antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16821-1-AP

  IP result of anti-SRF (IP:16821-1-AP, 3ug; Detection:16821-1-AP 1:1000) with HeLa cells lysate 3000ug.

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  IHC staining of mouse heart using 16821-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16821-1-AP (SRF antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 16821-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16821-1-AP (SRF antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 16821-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using SRF antibody (16821-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: A431 cells, MCF-7 cells, K-562 cells, HEK-293 cells, HeLa cells, NIH/3T3 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 16 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below
• ChIP: See 2 publications below

Product Information

16821-1-AP targets SRF in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SRF fusion protein Ag10386
• Full Name: serum response factor (c-fos serum response element-binding transcription factor)
• Calculated Molecular Weight: 508 aa, 52 kDa
• Observed Molecular Weight: 65-70 kDa, 40 kDa
• GenBank Accession Number: BC048211
• Gene Symbol: SRF
• Gene ID (NCBI): 6722
• RRID: AB_2194384
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF is required for cardiac differentiation and maturation due to the role in cardiac cell growth and muscle gene regulation. The full-length SRF protein is 67 kDa and molecular weight of cleaved fragment is 50 kDa and 20 kDa (PMID: 21769134).

Publications

Species	Application	Title
human	WB	Exp Mol Med; Helicobacter pylori CagA-mediated ether lipid biosynthesis promotes ferroptosis susceptibility in gastric cancer; Authors - Yanmei Peng ; KD Validated
human	IP	Am J Respir Cell Mol Biol; SRF Expression in Excess Permits a Dual Contractile-Proliferative Phenotype of Airway Smooth Muscle; Authors - Rui Sun
	WB	Cell Physiol Biochem; Galectin-3- Mediated Transdifferentiation of Pulmonary Artery Endothelial Cells Contributes to Hypoxic Pulmonary Vascular Remodeling.; Authors - Li Zhang
mouse	WB	Stem Cell Res Ther; Activin B-activated Cdc42 signaling plays a key role in regulating adipose-derived mesenchymal stem cells-mediated skin wound healing.; Authors - Simin Huang
human	WB	Am J Physiol Lung Cell Mol Physiol; Myocardin regulates fibronectin expression and secretion from human pleural mesothelial cells; Authors - Tsuyoshi Sakai
mouse	ChIP	J Immunol; TPL-2 Inhibits IFN-β Expression via an ERK1/2-TCF-FOS Axis in TLR4-Stimulated Macrophages.; Authors - Louise Blair

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Carmen (Verified Customer) (11-29-2024)

Works well IHC set up on the Leica Bond

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Murine pancreatic tumour