NR5A1 Polyclonal antibody

NR5A1 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 18658-1-AP

Synonyms

FTZF1, FTZ1, ELP, Adrenal 4-binding protein, Adrenal 4 binding protein

---

Validation Data Gallery

• 

  WB analysis using 18658-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18658-1-AP (NR5A1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 18658-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 18658-1-AP (NR5A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A2780 using 18658-1-AP

  IP result of anti-NR5A1 (IP:18658-1-AP, 4ug; Detection:18658-1-AP 1:300) with A2780 cells lysate 960ug.

• 

  IHC staining of human ovary using 18658-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 18658-1-AP (NR5A1 Antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of rat testis using 18658-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 18658-1-AP (NR5A1 Antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 18658-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 18658-1-AP (NR5A1 Antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 18658-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 18658-1-AP (NR5A1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 18658-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 18658-1-AP (NR5A1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 18658-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 18658-1-AP (NR5A1 Antibody) at dilution of 1:100 (under 10x lens).

• 

  FC experiment of HepG2 using 18658-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human NR5A1 (18658-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Tested Applications

• Positive WB detected in: mouse ovary tissue, rat ovary tissue
• Positive IP detected in: A2780 cells
• Positive IHC detected in: human ovary tissue, human liver tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 5 publications below
• IF: See 5 publications below

Product Information

18658-1-AP targets NR5A1 in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, goat, camel, ondatra zibethicus
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NR5A1 fusion protein Ag13252
• Full Name: nuclear receptor subfamily 5, group A, member 1
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC032501
• Gene Symbol: NR5A1
• Gene ID (NCBI): 2516
• RRID: AB_10596916
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Steroidogenic factor-1 (SF-1,STF-1), also known as NR5A1, regulates multiple genes involved in the adrenal and gonadal development and in the biosynthesis of a variety of hormones, including adrenal and gonadal steroids, anti-Mullerian hormone (AMH), and gonadotropins. SF-1 belongs to the fushi tarazu factor-1 (FTZ-F1) subfamily of orphan nuclear receptors. Initially identified as a tissue-specific transcriptional regulator of cytochrome P450 steroid hydroxylases, research studies of both global and tissue-specific knockout mice have demonstrated that SF-1 is required for the development of adrenal glands, gonads, ventromedial hypothalamus, and for the proper functioning of pituitary gonadotropes. Indeed, humans with mutations that render SF-1 transcriptionally inactive can present with testicular failure, ovarian failure, and adrenal insufficiency. Furthermore, dysregulation of SF-1 has been linked to diseases such as endometriosis and adrenocortical carcinoma.Like other nuclear hormone receptors, SF-1 has a modular domain structure composed of an N-terminal zinc finger DNA-binding domain, a ligand-binding domain, a C-terminal AF-2 activation domain, and a hinge region with AF-1-like activation activity. SF-1 also contains a fushi tarazu factor 1 box, which functions as an accessory DNA binding domain. SF-1 is primarily phosphorylated at Ser203, which is thought to enhance its transcriptional activity by promoting complex formation with transcriptional cofactors. In addition to phosphorylation at Ser203, SF-1 is subject to SUMO conjugation and acetylation at ε-amino groups of target lysine residues. Whereas SUMOylation represses SF-1 function, acetylation enhances its transcriptional activity.In the adult ovary, SF-1 localizes to theca/interstitial cells.

Publications

Species	Application	Title
rat	WB	J Hazard Mater; GRP78/IRE1 and cGAS/STING pathway crosstalk through CHOP facilitates iodoacetic acid-mediated testosterone decline; Authors - Li Mou
human	IF	Elife; Induction of Sertoli-like cells from human fibroblasts by NR5A1 and GATA4.; Authors - Jianlin Liang
mouse	WB,IF	Elife; PRMT5 regulates ovarian follicle development by facilitating Wt1 translation; Authors - Min Chen
mouse	IHC	Mol Cell Endocrinol; Pde8b haploinsufficiency in mice is associated with modest adrenal defects, impaired steroidogenesis, and male infertility, unaltered by concurrent PKA or Wnt activation.; Authors - Leticia Ferro Leal
Ondatra zibethicus	IHC	J Steroid Biochem Mol Biol; Seasonal expressions of SF-1, StAR and P450scc in the scent glands of the muskrats (Ondatra zibethicus).; Authors - Wenqian Xie
mouse	IF	Toxicol Appl Pharmacol; PMID: 31175881; Authors - Jiandong Sun