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  • KD/KO Validated

NKX2-5 Polyclonal antibody

NKX2-5 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (1)





Cat no : 13921-1-AP


Cardiac specific homeobox, CHNG5, CSX, CSX1, Homeobox protein CSX, Homeobox protein Nkx 2.5, NKX2 5, NKX2.5, NKX2-5, NKX2E, NKX4 1

Tested Applications

Positive WB detected inhuman heart tissue
Positive IHC detected inhuman brain tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse embryo tissue

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13921-1-AP targets NKX2-5 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NKX2-5 fusion protein Ag4930
Full Name NK2 transcription factor related, locus 5 (Drosophila)
Calculated Molecular Weight 324 aa, 35 kDa
Observed Molecular Weight30-42 kda
GenBank Accession NumberBC025711
Gene Symbol NKX2-5
Gene ID (NCBI) 1482
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development [PMID: 7665173]. NKX2-5 encoded by homeobox-containing genes that implicated in commitment to and/or differentiation of the myocardial lineage, and acts as a transcriptional activator of ANF in cooperation with GATA4. During embryonic development, NKX2-5 plays pivotal roles in specifying cardiac progenitors, cardiac morphogenesis, cardiomyocyte differentiation and conduction system development [PMID: 22824467].


Product Specific Protocols
WB protocol for NKX2-5 antibody 13921-1-APDownload protocol
IHC protocol for NKX2-5 antibody 13921-1-APDownload protocol
IF protocol for NKX2-5 antibody 13921-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Rep

Post-transcriptional Regulation of Nkx2-5 by RHAU in Heart Development.

Authors - Junwei Nie


A dual SHOX2:GFP; MYH6:mCherry knockin hESC reporter line for derivation of human SAN-like cells.

Authors - Zaniar Ghazizadeh

Am J Physiol Heart Circ Physiol

Homocysteine downregulates cardiac homeobox transcription factor NKX2.5 via IGFBP5.

Authors - Guangrui Lai

J Appl Toxicol

The effect of maternal exposure to di-(2-ethylhexyl)-phthalate on fetal cardiac development in mice.

Authors - Changqing Tang

Mol Med Rep

H3K9me2 regulates early transcription factors to promote mesenchymal stem‑cell differentiation into cardiomyocytes.

Authors - Xiaolin Sun

Int J Clin Exp Pathol

SDF-1α promotes repair of myocardial ischemic necrosis zones in rats.

Authors - Xinhua Cai


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Rebecca (Verified Customer) (10-25-2022)

20ug of protein were loaded. Transference was performed at 4ºC and 90V for 2h. Antibody incubation was done ON at 4ºC.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: iPSC-CM
NKX2-5 Antibody Western Blot validation (1:1000 dilution) in iPSC-CM (Cat no:13921-1-AP)