Anticorps Polyclonal de lapin anti-FSTL1

• Phare
• Validé par KD/KO

FSTL1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 20182-1-AP

Synonymes

follistatin like 1, Follistatin like protein 1, Follistatin related protein 1, FRP, FSL1, FSTL1, TSC-36

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Galerie de données de validation

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  WB analysis using 20182-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20182-1-AP (FSTL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 20182-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20182-1-AP (FSTL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20182-1-AP

  WB result of FSTL1 antibody (20182-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FSTL1 transfected HeLa cells.

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  WB analysis of human placenta using 20182-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 20182-1-AP (FSTL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A2780 using 20182-1-AP

  IP result of anti-FSTL1 (IP:20182-1-AP, 4ug; Detection:20182-1-AP 1:2000) with A2780 cells lysate 960 ug.

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  IHC staining of human placenta using 20182-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20182-1-AP (FSTL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 20182-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20182-1-AP (FSTL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 20182-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20182-1-AP (FSTL1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 20182-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20182-1-AP (FSTL1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 20182-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 20182-1-AP (FSTL1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 20182-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 20182-1-AP (FSTL1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of rat colon using 20182-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 20182-1-AP (FSTL1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of rat colon using 20182-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 20182-1-AP (FSTL1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of rat testis using 20182-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 20182-1-AP (FSTL1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of rat testis using 20182-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 20182-1-AP (FSTL1 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules A2780, cellules A431, cellules C6, cellules HeLa, tissu placentaire humain
• Résultats positifs en IP: cellules A2780,
• Résultats positifs en IHC: tissu placentaire humain, tissu de côlon de rat, tissu de gliome humain, tissu pancréatique humain, tissu testiculaire de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 10 publications below
• IHC: See 8 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

20182-1-AP cible FSTL1 dans les applications de WB, IP, IF, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FSTL1 Protéine recombinante Ag14057
• Nom complet: follistatin-like 1
• Masse moléculaire calculée: 308 aa, 35 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC000055
• Symbole du gène: FSTL1
• Identification du gène (NCBI): 11167
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Follistatin-like 1 (FSTL1), initially discovered as a TGF-β1-induced gene, encodes a 308 amino acid secreted 45-55 kDa glycoprotein with a follistatin domain and two non-functional calcium-binding motifs. FSTL1 has been reported to be involved in the fate determination and maturation of epithelial cells. Ablation of the FSTL1 gene in the mouse results in several structural developmental defects and neonatal lethality due to respiratory failure, demonstrating its functional importance. FSTL1 has been reported to exhibit both pro- and anti-inflammatory actions, with a specific anti-apoptotic and protective effect in cardiac or renal cell injury. Zwijsen et al. (1994) detected several isoforms of FSTL1 with molecular masses of 40 to 48 kD which differs from the 50- to 55-kD products detected by Tanaka et al. (1998) (OMIM).

Publications

Species	Application	Title
human	WB,IF	J Exp Med; EGF hijacks miR-198/FSTL1 wound-healing switch and steers a two-pronged pathway toward metastasis.; Authors - Gopinath M Sundaram
mouse	IHC	Bone Res; Single cell analysis reveals inhibition of angiogenesis attenuates the progression of heterotopic ossification in Mkx-/- mice.; Authors - Junxin Lin
human	WB	Cancer Res; Follistatin-like Protein 1 Inhibits Lung Cancer Metastasis by Preventing Proteolytic Activation of Osteopontin.; Authors - Jean Chiou ; KD Validated
human,mouse	WB,IHC,IP	Cell Death Dis; FSTL1 interacts with VIM and promotes colorectal cancer metastasis via activating the focal adhesion signalling pathway.; Authors - Chuansha Gu
mouse	IHC	Aging (Albany NY); Follistatin-like 1 deficiency impairs T cell development to promote lung metastasis of triple negative breast cancer.; Authors - Jie Ma ; KO Validated
human	IHC,CoIP	Am J Cancer Res; FSTL1 promotes growth and metastasis in gastric cancer by activating AKT related pathway and predicts poor survival.; Authors - Mengjie Wu ; KD Validated

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