Noggin Polyclonal antibody

Noggin Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 14772-1-AP

Synonyms

NOG, Noggin, SYM1, SYNS1

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Validation Data Gallery

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  WB analysis of HeLa using 14772-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14772-1-AP (Noggin antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 14772-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14772-1-AP (Noggin antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 14772-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14772-1-AP (Noggin antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

14772-1-AP targets Noggin in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Noggin fusion protein Ag6586
• Full Name: noggin
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC034027
• Gene Symbol: Noggin
• Gene ID (NCBI): 9241
• RRID: AB_2151671
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Noggin is an extracellular polypeptide acting as an antagonist of bone morphogenetic proteins (BMPs) regulating embryonal development. Noggin inhibits activity of BMP-2, -4, -7, -13, and -14.

What is the molecular weight of Noggin? Is Noggin post-translationally modified?

The molecular weight of Noggin is 26 kDa. Noggin is secreted as a covalently linked glycosylated homodimer (PMID: 12478285).

What is the subcellular localization of Noggin?

Noggin is present extracellularly in the matrix or retained at the cell surface via interaction with HSPG (PMID: 11706034).

What is the tissue expression pattern of Noggin?

In early development stages, Noggin is produced by the Spemann organizer, allowing dorsal-ventral patterning of BMPs (PMID: 8752214). Subsequently, Noggin is expressed by the notochord regulating BMP-4 signaling in neurogenesis. Additionally, Noggin is present during development in the dermal papilla, connective tissue of the hair follicle, lens, retina, and periocular mesenchyme, as well as in the mesoderm lineage regulating development of the bone, cartilage, and muscles (PMID: 21256973).

What is the use of Noggin in cell culture?

Noggin can be used for maintaining the pluripotency of human embryonic stem cells (PMID: 19500111).

Publications

Species	Application	Title
mouse	WB	Cell Death Dis; PMID: 29415997; Authors - Shu-Fen Li
hamster	IHC	Birth Defects Res; Cigarette smoke induced neural tube defects by down-regulating noggin expression.; Authors - Chuanhui Yin
mouse		Microbiome; Desulfovibrio vulgaris interacts with novel gut epithelial immune receptor LRRC19 and exacerbates colitis; Authors - Runxiang Xie