GREM2 Polyclonal antibody

GREM2 Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 13892-1-AP

Synonyms

Gremlin2, Gremlin 2, GREM 2, DAND3, DAND 3

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Validation Data Gallery

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  IHC staining of mouse ovary using 13892-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 13892-1-AP (GREM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 13892-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 13892-1-AP (GREM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 13892-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13892-1-AP (GREM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 13892-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13892-1-AP (GREM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 13892-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13892-1-AP (GREM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary using 13892-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 13892-1-AP (GREM2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 13892-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 13892-1-AP (GREM2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 13892-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13892-1-AP (GREM2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 13892-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13892-1-AP (GREM2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse kidney using 13892-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using GREM2 antibody (13892-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse ovary using 13892-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using GREM2 antibody (13892-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: mouse ovary tissue, human ovary tissue, human kidney tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse kidney tissue, mouse ovary tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Product Information

13892-1-AP targets GREM2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GREM2 fusion protein Ag4854
• Full Name: gremlin 2, cysteine knot superfamily, homolog (Xenopus laevis)
• Calculated Molecular Weight: 168 aa, 19 kDa
• GenBank Accession Number: BC046632
• Gene Symbol: GREM2
• Gene ID (NCBI): 64388
• RRID: AB_2877984
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IHC	Am J Pathol; The Onset of Heat-Induced Testicular Calcification in Mice: Involvement of the Telomeric Locus on Chromosome 1.; Authors - Masataka Chihara
human	IF	Stem Cells Dev; Coordinated proliferation and differentiation of human iPS cell-derived cardiac progenitor cells depends on BMP signaling regulation by Gremlin 2.; Authors - Jeffery Bylund
mouse	IHC	Andrology; Genetic factors derived from the MRL/MpJ mouse function to maintain the integrity of spermatogenesis after heat exposure.; Authors - M Chihara
mouse	WB,IHC	Metabolism; SGLT2 inhibitor empagliflozin downregulates miRNA-34a-5p and targets GREM2 to inactivate hepatic stellate cells and ameliorate non-alcoholic fatty liver disease-associated fibrosis; Authors - Yunfeng Shen
mouse	IF	Angiogenesis; Site-specific genetic and functional signatures of aortic endothelial cells at aneurysm predilection sites in healthy and AngII ApoE-/- mice; Authors - Alexander Brückner