Anticorps Polyclonal de lapin anti-FIS1

• Phare
• Validé par KD/KO

FIS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris et plus (6)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

288

Avis (4)

N° de cat : 10956-1-AP

Synonymes

TTC11, TPR repeat protein 11, hFis1, FIS1 homolog, CGI-135

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Galerie de données de validation

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  WB analysis using 10956-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10956-1-AP (FIS1 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa cells using 10956-1-AP

  WB result of FIS1 antibody (10956-1-AP, 1:8,000) with si-Control and si-FIS1 transfected HeLa cells.

• 

  WB analysis using 10956-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10956-1-AP (FIS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10956-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10956-1-AP (FIS1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 10956-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10956-1-AP (FIS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 10956-1-AP

  pig brain tissue were subjected to SDS PAGE followed by western blot with 10956-1-AP (FIS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10956-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10956-1-AP (FIS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 10956-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 10956-1-AP (FIS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10956-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10956-1-AP (FIS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 10956-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10956-1-AP (FIS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10956-1-AP

  IP result of anti-FIS1 (IP:10956-1-AP, 4ug; Detection:10956-1-AP 1:10000) with HeLa cells lysate 1120 ug.

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  IHC staining of rat brain using 10956-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10956-1-AP (FIS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 10956-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 10956-1-AP (FIS1 antibody) at dilution of 1:25 (under 40x lens).

• 

  IF Staining of Hepa1-6 cells using 10956-1-AP

  IF result of anti-FIS1(10956-1-AP,1:100) with Hepa1-6 cell by Dr. Steven Eugene Smith. Mitochondrion outer membrane (Green) Stain.

• 

  IF Staining of HepG2 using 10956-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10956-1-AP (FIS1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 10956-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FIS1 antibody (10956-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules PC-12, cellules SH-SY5Y, tissu cardiaque de rat, tissu cardiaque de souris, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris, tissu splénique de rat, tissu splénique de souris
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu cérébral de rat, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules Hepa1-6, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:14000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 10 publications below
• WB: See 267 publications below
• IHC: See 7 publications below
• IF: See 34 publications below
• IP: See 4 publications below
• CoIP: See 1 publications below

Informations sur le produit

10956-1-AP cible FIS1 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, bovin, Chèvre, Humain, poisson-zèbre, porc, poulet, souris, Hamster, squirrel
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FIS1 Protéine recombinante Ag1409
• Nom complet: fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae)
• Masse moléculaire calculée: 17 kDa
• Poids moléculaire observé: 17 kDa
• Numéro d’acquisition GenBank: BC009428
• Symbole du gène: FIS1
• Identification du gène (NCBI): 51024
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fis1 (fission 1) is an integral mitochondrial outer membrane protein that participates in mitochondrial fission by interacting with dynamin-related protein 1 (Drp1). Excessive mitochondrial fission is associated with the pathology of a number of neurodegenerative or neurodevelopmental diseases. Increased expression of Fis1 has been found in Huntington's disease (HD)-affected brain, Alzheimer's disease (AD) patients, and autism spectrum disorder. This antibody was raised against the full-length of human Fis1 protein, and recognizes endogenous Fis1 protein in various lysates. (PMID: 21257639, 21459773, 23333625)

Publications

Species	Application	Title
human	WB	Nature; Restoring metabolism of myeloid cells reverses cognitive decline in ageing.; Authors - Paras S Minhas
	WB,IF	Nature; Distinct fission signatures predict mitochondrial degradation or biogenesis.; Authors - Tatjana Kleele ; KO Validated
human	WB	Nat Immunol; RNA viruses promote activation of the NLRP3 inflammasome through a RIP1-RIP3-DRP1 signaling pathway.; Authors - Xiaqiong Wang ; KD Validated
human	WB,IF	Cell Stem Cell; AMPK/FIS1-Mediated Mitophagy Is Required for Self-Renewal of Human AML Stem Cells.; Authors - Shanshan Pei ; KD Validated
human	WB	Cell Metab; Fatty Liver Disease Caused by High-Alcohol-Producing Klebsiella pneumoniae.; Authors - Jing Yuan
mouse	WB,IP	Nat Metab; Mitochondrial fission drives neuronal metabolic burden to promote stress susceptibility in male mice; Authors - Wan-Ting Dong

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Megan (Verified Customer) (06-22-2021)

RPE cell over-expressing pcDNA hFis1. Note 1:500 was used here to detect over-expressed Fis1, but we also use 1:100 when detecting endogenous Fis1. Also successfully used to detect endogenous Fis1 levels via Western Blot (used @ 1:1000).

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:100 - 1:500
• Cell Tissue Type: RPE

FIS1 Antibody Western Blot,Immunofluorescence validation (1:100 - 1:500 dilution) in RPE (Cat no:10956-1-AP)

FH

Siting (Verified Customer) (01-28-2020)

This antibody worked perfectly for western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart tissue

FH

Chun (Verified Customer) (07-03-2019)

This antibody is excellent/

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2,000
• Cell Tissue Type: HEK293 and HeLa cells, and primary rat cortical neurons

FH

Kishor (Verified Customer) (12-13-2018)

I got good results in both rat liver protein and H69 cells.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: H69, HUCCT-1

FIS1 Antibody Western Blot, validation (1:1000 dilution) in H69, HUCCT-1 (Cat no:10956-1-AP)

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