|Positive WB detected in||HSC-T6 cells, T-47D cells, mouse brain tissue, mouse kidney tissue, mouse liver tissue|
|Positive IHC detected in||human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
13798-1-AP targets MFN1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, Chicken, ducks, monkey, hamster, bovine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MFN1 fusion protein Ag4762|
|Full Name||mitofusin 1|
|Calculated molecular weight||741 aa, 84 kDa|
|Observed molecular weight||86 kDa|
|GenBank accession number||BC040557|
|Gene ID (NCBI)||55669|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Mitofusin-1 (MFN1) is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). Mitofusins are large predicted GTPases located in outer mitochondrial membrane. They are essential for outer membrane fusion by interacting with each other to facilitate mitochondrial targeting. The mitofusins are the first known protein mediator of mitochondrial fusion, and mediate developmentally regulated post-meiotic fusion of mitochondria.Mfn1 is required on adjacent mitochondria to mediate fusion via interactions of a heptad repeat region that mediates oligomerization of the protein(PMID:16892085). Mitofusin 1 and mitofusin 2 are ubiquitinated in a PINK1/parkin-dependent manner upon induction of mitophagy(PMID: 20871098).
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Ruiting (Verified Customer) (09-18-2020)
Always purchase antibodies from your company, antibody is cheap, and works well. Never has a bad experience in any antibody. Plan to buy two more next week.
Kishor (Verified Customer) (01-30-2019)
I working good for Western Blotting (1:1000) in human cancer cells but not for rat liver tissue