MFN1 Polyclonal antibody

• Featured Product
• KD/KO Validated

MFN1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

263

Reviews (2)

Cat no : 13798-1-AP

Synonyms

Mitofusin-1, mitofusin 1, hfzo2, hfzo1, Fzo homolog

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Validation Data Gallery

• 

  WB analysis using 13798-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13798-1-AP (MFN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 13798-1-AP

  IP result of anti-MFN1 (IP:13798-1-AP, 4ug; Detection:13798-1-AP 1:5000) with mouse kidney tissue lysate 2160 ug.

• 

  IHC staining of human kidney using 13798-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13798-1-AP (MFN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 13798-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using MFN1 antibody (13798-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HSC-T6 cells, T-47D cells, mouse brain tissue, mouse kidney tissue, mouse liver tissue
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 251 publications below
• IHC: See 13 publications below
• IF: See 13 publications below
• IP: See 1 publications below

Product Information

13798-1-AP targets MFN1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, monkey, chicken, hamster, goat, ducks, duck
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MFN1 fusion protein Ag4762
• Full Name: mitofusin 1
• Calculated Molecular Weight: 741 aa, 84 kDa
• Observed Molecular Weight: 86 kDa
• GenBank Accession Number: BC040557
• Gene Symbol: MFN1
• Gene ID (NCBI): 55669
• RRID: AB_2266318
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mitofusin-1 (MFN1) is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). Mitofusins are large predicted GTPases located in outer mitochondrial membrane. They are essential for outer membrane fusion by interacting with each other to facilitate mitochondrial targeting. The mitofusins are the first known protein mediator of mitochondrial fusion, and mediate developmentally regulated post-meiotic fusion of mitochondria.Mfn1 is required on adjacent mitochondria to mediate fusion via interactions of a heptad repeat region that mediates oligomerization of the protein(PMID:16892085). Mitofusin 1 and mitofusin 2 are ubiquitinated in a PINK1/parkin-dependent manner upon induction of mitophagy(PMID: 20871098).

Publications

Species	Application	Title
human	WB	Cell Res; Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression; Authors - Shiqiao Qiu
mouse	WB	Cell; Mitocytosis, a migrasome-mediated mitochondrial quality-control process.; Authors - Haifeng Jiao ; KD Validated
human	WB	Cell Metab; Fatty Liver Disease Caused by High-Alcohol-Producing Klebsiella pneumoniae.; Authors - Jing Yuan
mouse	WB	Cell Metab; Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.; Authors - Xiuying Zhong
mouse	WB	ACS Cent Sci; Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury; Authors - Juan Zhang
mouse	WB	Mol Cell; Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation; Authors - Chuanlong Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ruiting (Verified Customer) (09-18-2020)

Always purchase antibodies from your company, antibody is cheap, and works well. Never has a bad experience in any antibody. Plan to buy two more next week.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse tissues

FH

Kishor (Verified Customer) (01-30-2019)

I working good for Western Blotting (1:1000) in human cancer cells but not for rat liver tissue

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: H69, HUCCT-1

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