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  • KD/KO Validated

MFN1 Polyclonal antibody

MFN1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB,IP,IF,IHC,ELISA

Conjugate

Unconjugated

Cat no : 13798-1-AP

Synonyms

Fzo homolog, hfzo1, hfzo2, MFN1, mitofusin 1, Transmembrane GTPase MFN1



Tested Applications

Positive WB detected inHSC-T6 cells, T-47D cells, mouse brain tissue, mouse kidney tissue, mouse liver tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13798-1-AP targets MFN1 in WB,IP,IF,IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, Chicken, ducks, monkey, hamster, pig, duck, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MFN1 fusion protein Ag4762
Full Name mitofusin 1
Calculated Molecular Weight 741 aa, 84 kDa
Observed Molecular Weight 86 kDa
GenBank Accession NumberBC040557
Gene Symbol MFN1
Gene ID (NCBI) 55669
RRIDAB_2266318
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mitofusin-1 (MFN1) is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). Mitofusins are large predicted GTPases located in outer mitochondrial membrane. They are essential for outer membrane fusion by interacting with each other to facilitate mitochondrial targeting. The mitofusins are the first known protein mediator of mitochondrial fusion, and mediate developmentally regulated post-meiotic fusion of mitochondria.Mfn1 is required on adjacent mitochondria to mediate fusion via interactions of a heptad repeat region that mediates oligomerization of the protein(PMID:16892085). Mitofusin 1 and mitofusin 2 are ubiquitinated in a PINK1/parkin-dependent manner upon induction of mitophagy(PMID: 20871098).

Protocols

Product Specific Protocols
WB protocol for MFN1 antibody 13798-1-APDownload protocol
IHC protocol for MFN1 antibody 13798-1-APDownload protocol
IF protocol for MFN1 antibody 13798-1-APDownload protocol
IP protocol for MFN1 antibody 13798-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Res

Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression

Authors - Shiqiao Qiu
mouseWB

Cell

Mitocytosis, a migrasome-mediated mitochondrial quality-control process.

Authors - Haifeng Jiao
  • KD Validated
humanWB

Cell Metab

Fatty Liver Disease Caused by High-Alcohol-Producing Klebsiella pneumoniae.

Authors - Jing Yuan
mouseWB

Cell Metab

Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.

Authors - Xiuying Zhong
mouseWB

ACS Cent Sci

Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury

Authors - Juan Zhang
mouseWB

Mol Cell

Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation

Authors - Chuanlong Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ruiting (Verified Customer) (09-18-2020)

Always purchase antibodies from your company, antibody is cheap, and works well. Never has a bad experience in any antibody. Plan to buy two more next week.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse tissues
FH

Kishor (Verified Customer) (01-30-2019)

I working good for Western Blotting (1:1000) in human cancer cells but not for rat liver tissue

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: H69, HUCCT-1