Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, mouse brain tissue, DU 145 cells, HCT 116 cells, HepG2 cells, NIH/3T3 cells, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12957-1-AP targets DRP1 (C-terminal) in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey, chicken, bovine, hamster, goat, duck |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3644 Product name: Recombinant human DNM1L,DLP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 361-711 aa of BC024590 Sequence: CGGARICYIFHETFGRTLESVDPLGGLNTIDILTAIRNATGPRPALFVPEVSFELLVKRQIKRLEEPSLRCVELVHEEMQRIIQHCSNYSTQELLRFPKLHDAIVEVVTCLLRKRLPVTNEMVHNLVAIELAYINTKHPDFADACGLMNNNIEEQRRNRLARELPSAVSRDKLIQDSRRETKNVASGGGGVGDGVQEPTTGNWRGMLKTSKAEELLAEEKSKPIPIMPASPQKGHAVNLLDVPVPVARKLSAREQRDCEVIERLIKSYFLIVRKNIQDSVPKAVMHFLVNHVKDTLQSELVGQLYKSSLLDDLLTESEDMAQRRKEAADMLKALQGASQIIAEIRETHLW Predict reactive species |
| Full Name | dynamin 1-like |
| Calculated Molecular Weight | 736 aa, 82 kDa |
| Observed Molecular Weight | 75-80 kDa |
| GenBank Accession Number | BC024590 |
| Gene Symbol | DRP1 |
| Gene ID (NCBI) | 10059 |
| RRID | AB_2093525 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O00429 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DNM1L(Dynamin-1-like protein) , also known as dynamin-related protein 1 (DRP1), belongs to the dynamin family of large GTPases that mediate membrane remodeling during a variety of cellular processes. DNM1L has an important role in the division of growing mitochondria and peroxisomes and also mediates outer mitochondrial membrane fission in mammalian cells. DNM1L is ubiquitously expressed with abundant expression in skeletal muscle, heart, kidney and brain.Variable isoforms of DNM1L have been reported in a tissue-specific manner due to the alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for DRP1 (C-terminal) antibody 12957-1-AP | Download protocol |
| IF protocol for DRP1 (C-terminal) antibody 12957-1-AP | Download protocol |
| IHC protocol for DRP1 (C-terminal) antibody 12957-1-AP | Download protocol |
| IP protocol for DRP1 (C-terminal) antibody 12957-1-AP | Download protocol |
| WB protocol for DRP1 (C-terminal) antibody 12957-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-DRP1 antibody (Cat# 12957-1-AP) has accumulated 521 citations across high-impact journals including Nature Communications, Cell Research, Cell Metabolism, Signal Transduction and Targeted Therapy, Autophagy, Redox Biology, Free Radical Biology and Medicine, Theranostics, Cell Death & Disease, and Journal of Clinical Investigation. Research fields span mitochondrial dynamics and fission, mitophagy, ferroptosis, cancer biology, neurodegeneration, cardiovascular disease, diabetes, renal injury, hepatic disorders, and inflammatory diseases.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Protein C receptor maintains cancer stem cell properties via activating lipid synthesis in nasopharyngeal carcinoma. | ||
Signal Transduct Target Ther Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy | ||
Cell Res Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression | ||
Bioact Mater Profibrogenic macrophage-targeted delivery of mitochondrial protector via exosome formula for alleviating pulmonary fibrosis
| ||
Bioact Mater Copper doped bioactive glass promotes matrix vesicles-mediated biomineralization via osteoblast mitophagy and mitochondrial dynamics during bone regeneration |
Reviews
What customers say
Overall, customers rate this antibody highly (average 4.4/5 across 5 reviews). Most users applied it for Western Blot at a 1:1000 dilution, with positive results in HEK cells, human white adipocytes, C2C12 cell line, and T cells. One user also used it for immunofluorescence on HeLa cells at 1:100 with paraformaldehyde fixation and Triton X-100 permeabilization. The most common feedback was that the product "works well," with one reviewer simply noting "Good." No negative or critical comments were reported.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Pierre (Verified Customer) (09-26-2025) | Good
|
P (Verified Customer) (09-23-2024) | Very Good
 |
Mi (Verified Customer) (03-06-2023) | It works pretty well in human white adipocyte cells.
|
Samrajni (Verified Customer) (04-27-2022) | The product works well.
|
Tom (Verified Customer) (07-07-2021) | Paraformaldehyde fixation and Triton X-100 permeabilization.
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