Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human stomach tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17090-1-AP targets MFF in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, monkey, hamster, goat, squirrel |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9990 Product name: Recombinant human MFF protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-238 aa of BC000797 Sequence: MAEISRIQYEMEYTEGISQRMRVPEKLKVAPPNADLEQGFQEGVPNASVIMQVPERIVVAGNNEDVSFSRPADLDLIQSTPFKPLALKTPPRVLTLSERPLDFLDLERPPTTPQNEEIRAVGRLKRERSMSENAVRQNGQLVRNDSLPVLRGGSAAATSNPHHDNVRYGISNIDTTIEGTSDDLTVVDAASLRRQIIKLNRRLQLLEEENKERAKREMVMYSITVAFWLLNSWLWFRR Predict reactive species |
| Full Name | mitochondrial fission factor |
| Calculated Molecular Weight | 38 kDa |
| Observed Molecular Weight | 27-38 kDa |
| GenBank Accession Number | BC000797 |
| Gene Symbol | MFF |
| Gene ID (NCBI) | 56947 |
| RRID | AB_2142463 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9GZY8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Mitochondrial Fission Factor (MFF) is a critical regulator of organelle division, specifically playing a central role in the fission of mitochondria and peroxisomes. DRP1, MiD, and MFF form a trimeric complex in which MiD proteins facilitate the MFF-DRP1 interaction. Moreover, MiD51 can inhibit MFF-induced activation of DRP1 GTPase activity. Multiple isoforms of MFF exist due to the alternative splicing. This antibody recognizes the endogenous MFF protein around 26-29 kDa and 35-38 kDa.(PMID: 39365854, PMID: 37291333)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MFF antibody 17090-1-AP | Download protocol |
| IHC protocol for MFF antibody 17090-1-AP | Download protocol |
| IP protocol for MFF antibody 17090-1-AP | Download protocol |
| WB protocol for MFF antibody 17090-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Science Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division. | ||
Signal Transduct Target Ther Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy | ||
Cell CerS6-Derived Sphingolipids Interact with Mff and Promote Mitochondrial Fragmentation in Obesity. |
Reviews
What customers say
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Parmita (Verified Customer) (07-23-2025) | working very good
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Chun (Verified Customer) (09-07-2020) | This antibody is good for IP, and there are non-specific band(s) detected when it is used for immunoblotting.
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JAE HO (Verified Customer) (04-22-2019) | I used couple of antibodies for MFF proteins. Only this antibody was working nicely. I satisfied its higher titer and specificity. I have tested and used this antibody for WB and IP.
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