Anticorps Polyclonal de lapin anti-ERP29

ERP29 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 24344-1-AP

Synonymes

C12orf8, ERp28, ERP29, ERp31, PDI DB, PDIA9

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Galerie de données de validation

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  WB analysis using 24344-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24344-1-AP (ERP29 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 24344-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 24344-1-AP (ERP29 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 24344-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24344-1-AP (ERP29 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 24344-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24344-1-AP (ERP29 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 24344-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24344-1-AP (ERP29 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 24344-1-AP

  IP result of anti-ERP29 (IP:24344-1-AP, 4ug; Detection:24344-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

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  IHC staining of human liver cancer using 24344-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24344-1-AP (ERP29 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 24344-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24344-1-AP (ERP29 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 24344-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24344-1-AP (ERP29 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 24344-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24344-1-AP (ERP29 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 24344-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 24344-1-AP (ERP29 Antibody) at dilution of 1:50.

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  IHC staining of human pancreas using 24344-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 24344-1-AP (ERP29 Antibody) at dilution of 1:50.

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  IF Staining of HeLa using 24344-1-AP

  Immunofluorescent analysis of HeLa cells using 24344-1-AP (ERP29 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  IF Staining of human liver cancer using 24344-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ERP29 antibody (24344-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human liver cancer using 24344-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ERP29 antibody (24344-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules L02, cellules HEK-293, tissu hépatique de souris, tissu pulmonaire de souris, tissu rénal de souris, tissu testiculaire de rat
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du sein humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cancer du foie humain,
• Résultats positifs en IF/ICC: cellules HeLa, tissu de cancer du foie humain

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

24344-1-AP cible ERP29 dans les applications de WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ERP29 Protéine recombinante Ag19451
• Nom complet: endoplasmic reticulum protein 29
• Masse moléculaire calculée: 261 aa, 29 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: BC101495
• Symbole du gène: ERP29
• Identification du gène (NCBI): 10961
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Endoplasmic reticulum resident protein 29 (ERP29) is a 29-kDa endoplasmic reticulum (ER) luminal protein. Structurally ERp29 is related to protein disulfide isomerases (PDI). However, it lacks the thioredoxin motif, suggesting that this protein does not function as a disulfide isomerase (PMID: 15500441). ERP29 plays an important role in the processing of secretory proteins within the (ER), possibly by participating in the folding of proteins in the ER. The secretion of thyroglobulin (TG) was found to be strongly regulated by ERP29 (PMID: 16380091).

Publications

Species	Application	Title
mouse	WB	iScience; Non-canonical function of DPP4 promotes cognitive impairment through ERp29-associated mitochondrial calcium overload in diabetes; Authors - Jiaxiu Li
mouse	WB,IF,CoIP	Metabolism; Nonenzymatic function of DPP4 promotes diabetes-associated cognitive dysfunction through IGF-2R/PKA/SP1/ERp29/IP3R2 pathway-mediated impairment of Treg function and M1 microglia polarization; Authors - Ya Hui

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