ERp72 Polyclonal antibody

• Featured Product
• KD/KO Validated

ERp72 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

43

Cat no : 14712-1-AP

Synonyms

Protein disulfide-isomerase A4, PDIA4, ERp-72, ERp70, ERp 72

---

Validation Data Gallery

• 

  WB analysis using 14712-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14712-1-AP (ERp72 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14712-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14712-1-AP (ERp72 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14712-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14712-1-AP (ERp72 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14712-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14712-1-AP (PDIA4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14712-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14712-1-AP (PDIA4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 14712-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 14712-1-AP (PDIA4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14712-1-AP

  IP result of anti-ERp72 (IP:14712-1-AP, 4ug; Detection:14712-1-AP 1:500) with HeLa cells lysate 2800ug.

• 

  IHC staining of human liver cancer using 14712-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14712-1-AP (ERp72 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14712-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14712-1-AP (ERp72 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 14712-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14712-1-AP (ERp72 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 14712-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14712-1-AP (ERp72 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A431 using 14712-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using ERp72 antibody (14712-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of PC-3 using 14712-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using ERp72 antibody (14712-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  FC experiment of HepG2 using 14712-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human ERp72 (14712-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive WB detected in: mouse brain tissue, L02 cells, HeLa cells, HepG2 cells, mouse liver tissue, rat liver tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human liver cancer tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells, PC-3 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 30 publications below
• IHC: See 3 publications below
• IF: See 15 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

14712-1-AP targets ERp72 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ERp72 fusion protein Ag6431
• Full Name: protein disulfide isomerase family A, member 4
• Calculated Molecular Weight: 73 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC000425
• Gene Symbol: ERp72
• Gene ID (NCBI): 9601
• RRID: AB_2160973
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDIA4(Protein disulfide-isomerase A4) is also named as ERP70, ERP72 and belongs to the protein disulfide isomerase family. It catalyzes the rearrangement of -S-S- bonds in proteins. ERp72 is a soluble protein localized in the ER lumen and contains the COOH-terminal retention signal, KEEL. There are 6 cysteine residues in the amino acid sequences of mouse and human ERp72. All of the cysteine residues occur in the internal thioredoxin motif, CGHC(PMID:15475357). The full length protein has a signal peptide with 20 amino acid.

Publications

Species	Application	Title
mouse	WB	Cell; MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation.; Authors - Xiaorong Zhang
human	WB	J Thromb Haemost; SINE-VNTR-Alu retrotransposon insertion as a novel mutational event underlying Glanzmann thrombasthenia; Authors - Jiasheng Zhang
human	WB	Mol Syst Biol; Time-resolved interactome profiling deconvolutes secretory protein quality control dynamics; Authors - Madison T Wright
		Elife; Small molecule proteostasis regulators that reprogram the ER to reduce extracellular protein aggregation.; Authors - Lars Plate
human	WB	Cell Chem Biol; Quantitative Interactome Proteomics Reveals a Molecular Basis for ATF6-Dependent Regulation of a Destabilized Amyloidogenic Protein.; Authors - Lars Plate
mouse	WB	Elife; PDIA1/P4HB is required for efficient proinsulin maturation and ß cell health in response to diet induced obesity.; Authors - Insook Jang