Anticorps Polyclonal de lapin anti-DICER1

• Phare
• Validé par KD/KO

DICER1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

24

N° de cat : 20567-1-AP

Synonymes

HERNA, Helicase MOI, Endoribonuclease Dicer, EC:3.1.26.3, DICER

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Galerie de données de validation

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  WB analysis of HepG2 using 20567-1-AP

  WB result of DICER1 antibody (20567-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-DICER1 transfected HepG2 cells.

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  WB analysis of HeLa using 20567-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20567-1-AP (DICER1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 20567-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20567-1-AP (DICER1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 20567-1-AP

  IP result of anti-DICER1 (IP:20567-1-AP, 5ug; Detection:20567-1-AP 1:300) with Jurkat cells lysate 2000ug.

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  IHC staining of human testis using 20567-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20567-1-AP (DICER1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human lung cancer using 20567-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20567-1-AP (DICER1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 20567-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20567-1-AP (DICER1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 20567-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20567-1-AP (DICER1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 20567-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20567-1-AP (DICER1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 20567-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20567-1-AP (DICER1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IF Staining of human ovary tumor using 20567-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using DICER1 antibody (20567-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human ovary tumor using 20567-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using DICER1 antibody (20567-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 20567-1-AP

  Immunofluorescent analysis of HepG2 cells using 20567-1-AP (DICER1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  FC experiment of HepG2 using 20567-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human DICER1 (20567-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules K-562
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu testiculaire humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de tumeur ovarienne humain,
• Résultats positifs en IF/ICC: cellules HepG2
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 22 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Informations sur le produit

20567-1-AP cible DICER1 dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: dicer 1, ribonuclease type III
• Masse moléculaire calculée: 219 kDa
• Poids moléculaire observé: 220-250 kDa, 90 kDa
• Numéro d’acquisition GenBank: NM_030621
• Symbole du gène: DICER1
• Identification du gène (NCBI): 23405
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DICER1, also named as DICER, HERNA and KIAA0928, belongs to the helicase family and Dicer subfamily. It is required for formation of the RNA induced silencing complex (RISC). DICER1 is a component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. DICER1 cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs. The antibody is specific to DICER1. The calcualted molecular weight of DICER1 is 219 kDa, but modified DICER1 is about 220-250 kDa. DICER1 exists some isoforms with molecular weight 219 kDa and 93 kDa.

Publications

Species	Application	Title
	WB	Neuron; Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.; Authors - Xiaohui Li ; KD Validated
human	WB	Nat Chem Biol; Differential roles of human PUS10 in miRNA processing and tRNA pseudouridylation.; Authors - Jinghui Song
human	WB	Nat Commun; RNA structure probing reveals the structural basis of Dicer binding and cleavage.; Authors - Qing-Jun Luo
human	WB,IP	Nucleic Acids Res; HSP90 and Aha1 modulate microRNA maturation through promoting the folding of Dicer1.; Authors - Xiaochuan Liu
human	WB	Nat Commun; Long non-coding RNA GAS5 controls human embryonic stem cell self-renewal by maintaining NODAL signalling.; Authors - Chen Xu
human	WB	J Pathol; Cancer-associated somatic DICER1 hotspot mutations cause defective miRNA processing and reverse-strand expression bias to predominantly mature 3p strands through loss of 5p strand cleavage.; Authors - Anglesio M S MS ; KO Validated

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