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  • KD/KO Validated

TRBP Polyclonal antibody

TRBP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 15753-1-AP

Synonyms

TARBP2, TRBP1, Trans-activation-responsive RNA-binding protein, TAR RNA-binding protein 2, RISC-loading complex subunit TARBP2



Tested Applications

Positive WB detected inHepG2 cells, mouse liver tissue, mouse spleen tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman brain tissue, human kidney tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15753-1-AP targets TRBP in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRBP fusion protein Ag8255
Full Name TAR (HIV-1) RNA binding protein 2
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 43-46 kDa
GenBank Accession NumberBC005860
Gene Symbol TRBP
Gene ID (NCBI) 6895
RRIDAB_2200485
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TARBP2 is a component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC). Within the complex, TARBP2 and DICER1 are required to process precursor miRNAs (pre-miRNA) to mature miRNA and then load them onto EIF2C2/AGO2. It may has a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Otherwise, it binds to the HIV-1 TAR RNA that is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. The endogenous TRBP that was present in HEK293 cells, with apparent molecular masses of 43-46 kDa, are expressed in human cells. (PMID: 16142218)

Protocols

Product Specific Protocols
WB protocol for TRBP antibody 15753-1-APDownload protocol
IHC protocol for TRBP antibody 15753-1-APDownload protocol
IF protocol for TRBP antibody 15753-1-APDownload protocol
IP protocol for TRBP antibody 15753-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Mol Cell

Nuclear TARBP2 Drives Oncogenic Dysregulation of RNA Splicing and Decay.

Authors - Lisa Fish
  • KD Validated
mouseWB

Mol Cell

A Rapid Induction Mechanism for Lin28a in Trophic Responses.

Authors - Alexandra M Amen
humanWB

Nat Commun

Hypoxia regulates overall mRNA homeostasis by inducing Met1-linked linear ubiquitination of AGO2 in cancer cells.

Authors - Hailong Zhang
humanWB

Nat Commun

SUMOylation of TARBP2 regulates miRNA/siRNA efficiency.

Authors - Cheng Chen
  • KD Validated
humanWB,IF

Nucleic Acids Res

S6K2-mediated regulation of TRBP as a determinant of miRNA expression in human primary lymphatic endothelial cells.

Authors - Matthew J Warner
  • KD Validated
humanWB

J Med Chem

Identification of a Novel TAR RNA-Binding Protein 2 Modulator with Potential Therapeutic Activity against Hepatocellular Carcinoma.

Authors - Zongyuan Zhou