TRBP Polyclonal antibody

• Featured Product
• KD/KO Validated

TRBP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugate

Unconjugated

Publications

20

Cat no : 15753-1-AP

Synonyms

TARBP2, TRBP1, Trans-activation-responsive RNA-binding protein, TAR RNA-binding protein 2, RISC-loading complex subunit TARBP2

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Validation Data Gallery

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  WB analysis of HepG2 using 15753-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15753-1-AP (TRBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15753-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15753-1-AP (TRBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15753-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15753-1-AP (TRBP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 15753-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 15753-1-AP (TRBP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 15753-1-AP

  IP result of anti-TRBP (IP:15753-1-AP, 4ug; Detection:15753-1-AP 1:1500) with mouse liver tissue lysate 6400ug.

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  IHC staining of human brain using 15753-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15753-1-AP (TRBP antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 15753-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15753-1-AP (TRBP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 15753-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15753-1-AP (TRBP antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 15753-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15753-1-AP (TRBP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 15753-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15753-1-AP (TRBP antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 15753-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15753-1-AP (TRBP antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 15753-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15753-1-AP (TRBP antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 15753-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15753-1-AP (TRBP antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 15753-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15753-1-AP (TRBP antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 15753-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15753-1-AP (TRBP antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 15753-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15753-1-AP (TRBP antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 15753-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15753-1-AP (TRBP antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HeLa using 15753-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRBP antibody (15753-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse liver tissue, mouse spleen tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human brain tissue, human kidney tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 20 publications below
• IF: See 2 publications below
• IP: See 3 publications below
• RIP: See 1 publications below

Product Information

15753-1-AP targets TRBP in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRBP fusion protein Ag8255
• Full Name: TAR (HIV-1) RNA binding protein 2
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 43-46 kDa
• GenBank Accession Number: BC005860
• Gene Symbol: TRBP
• Gene ID (NCBI): 6895
• RRID: AB_2200485
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TARBP2 is a component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC). Within the complex, TARBP2 and DICER1 are required to process precursor miRNAs (pre-miRNA) to mature miRNA and then load them onto EIF2C2/AGO2. It may has a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Otherwise, it binds to the HIV-1 TAR RNA that is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. The endogenous TRBP that was present in HEK293 cells, with apparent molecular masses of 43-46 kDa, are expressed in human cells. (PMID: 16142218)

Publications

Species	Application	Title
human	WB,IF	Mol Cell; Nuclear TARBP2 Drives Oncogenic Dysregulation of RNA Splicing and Decay.; Authors - Lisa Fish ; KD Validated
mouse	WB	Mol Cell; A Rapid Induction Mechanism for Lin28a in Trophic Responses.; Authors - Alexandra M Amen
human	WB	Nat Commun; Hypoxia regulates overall mRNA homeostasis by inducing Met1-linked linear ubiquitination of AGO2 in cancer cells.; Authors - Hailong Zhang
human	WB	Nat Commun; SUMOylation of TARBP2 regulates miRNA/siRNA efficiency.; Authors - Cheng Chen ; KD Validated
human	WB,IF	Nucleic Acids Res; S6K2-mediated regulation of TRBP as a determinant of miRNA expression in human primary lymphatic endothelial cells.; Authors - Matthew J Warner ; KD Validated
human	WB	J Med Chem; Identification of a Novel TAR RNA-Binding Protein 2 Modulator with Potential Therapeutic Activity against Hepatocellular Carcinoma.; Authors - Zongyuan Zhou