Drosha Polyclonal antibody

• Featured Product
• KD/KO Validated

Drosha Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 55001-1-AP

Synonyms

DROSHA, ETOHI2, HSA242976, p241, RANSE3L, Ribonuclease 3, Ribonuclease III, ribonuclease type III, nuclear, RN3, RNase III, RNASE3L, RNASEN

---

Validation Data Gallery

• 

  WB analysis of HEK-293 using 55001-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55001-1-AP (Drosha antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 55001-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55001-1-AP (Drosha antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 55001-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55001-1-AP (Drosha antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 55001-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55001-1-AP (Drosha antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 55001-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55001-1-AP (Drosha antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 55001-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55001-1-AP (Drosha antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 55001-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55001-1-AP (Drosha antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55001-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55001-1-AP (Drosha antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below

Product Information

55001-1-AP targets Drosha in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: ribonuclease type III, nuclear
• Calculated Molecular Weight: 159 kDa
• Observed Molecular Weight: 90 kDa, 151 kDa
• GenBank Accession Number: NM_013235
• Gene Symbol: DROSHA
• Gene ID (NCBI): 29102
• RRID: AB_10859254
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DROSHA, also named as RN3, RNASE3L, P241 and RNASEN, is double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. It is a component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, RNASEN/DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. RNASEN is involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. And it is involved in the formation of GW bodies. The antibody is specific to DROSHA. DROSHA/RNASEN has some transcription forms with MW 160kd, 151kd, 138kd and 90kd.

Publications

Species	Application	Title
human	WB	Sci Rep; The Fragment HMGA2-sh-3p20 from HMGA2 mRNA 3'UTR Promotes the Growth of Hepatoma Cells by Upregulating HMGA2.; Authors - Yuan Wang
human	WB	Am J Physiol Cell Physiol; Hydrogen sulfide rescues high glucose-induced migration dysfunction in HUVECs by upregulating miR-126-3p.; Authors - Wen-Long Xue
human	WB	J Gen Virol; HBV induced the discharge of intrinsic antiviral miRNAs in HBV-replicating hepatocytes via extracellular vesicles to facilitate its replication.; Authors - Qiaofang Chu ; KD Validated
		Acta Pharmacol Sin; Post-transcriptional modulation of protein phosphatase PPP2CA and tumor suppressor PTEN by endogenous siRNA cleaved from hairpin within PTEN mRNA 3'UTR in human liver cells.; Authors - Yu-En Gao
human	WB	Cell Death Dis; FoxP3-miR-150-5p/3p suppresses ovarian tumorigenesis via an IGF1R/IRS1 pathway feedback loop.; Authors - Qinkai Zhang
human	WB	J Biol Chem; Zinc-finger protein YY1 suppresses tumor growth of human nasopharyngeal carcinoma by inactivating c-Myc-mediated microRNA-141 transcription.; Authors - Mengna Li