Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55001-1-AP targets DROSHA in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | ribonuclease type III, nuclear |
| Calculated Molecular Weight | 159 kDa |
| Observed Molecular Weight | 90 kDa, 151 kDa |
| GenBank Accession Number | NM_013235 |
| Gene Symbol | DROSHA |
| Gene ID (NCBI) | 29102 |
| RRID | AB_10859254 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NRR4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DROSHA, also named as RN3, RNASE3L, P241 and RNASEN, is double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. It is a component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, RNASEN/DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. RNASEN is involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. And it is involved in the formation of GW bodies. The antibody is specific to DROSHA. DROSHA/RNASEN has some transcription forms with MW 160kd, 151kd, 138kd and 90kd.
Publications
What published studies show
The DROSHA polyclonal antibody (Cat# 55001-1-AP) has 7 citations published in Cell Death & Disease, Acta Pharmacologica Sinica, American Journal of Physiology–Cell Physiology, Scientific Reports, Acta Biochimica et Biophysica Sinica, Journal of Biological Chemistry, and Journal of General Virology. Research fields span miRNA biogenesis, ovarian and hepatocellular carcinoma, nasopharyngeal carcinoma, vascular dysfunction, intestinal epithelial homeostasis, and HBV replication.
| Species | Application | Title |
|---|---|---|
Cell Death Dis FoxP3-miR-150-5p/3p suppresses ovarian tumorigenesis via an IGF1R/IRS1 pathway feedback loop. | ||
Acta Pharmacol Sin Post-transcriptional modulation of protein phosphatase PPP2CA and tumor suppressor PTEN by endogenous siRNA cleaved from hairpin within PTEN mRNA 3'UTR in human liver cells. | ||
Am J Physiol Cell Physiol Hydrogen sulfide rescues high glucose-induced migration dysfunction in HUVECs by upregulating miR-126-3p. | ||
Sci Rep The Fragment HMGA2-sh-3p20 from HMGA2 mRNA 3'UTR Promotes the Growth of Hepatoma Cells by Upregulating HMGA2. | ||
Acta Biochim Biophys Sin (Shanghai) 5'-tiRNA-Lys maintains intestinal epithelial homeostasis by EWSR1-dependent suppression of miR-125a and autophagy activation. | ||
J Biol Chem Zinc-finger protein YY1 suppresses tumor growth of human nasopharyngeal carcinoma by inactivating c-Myc-mediated microRNA-141 transcription. |
