Validation Data Gallery
|Positive WB detected in||human brain tissue, HEK293 cells, mouse kidney tissue, mouse spleen tissue, mouse thymus tissue, HEK-293 cells, mouse brain tissue, mouse heart tissue, HeLa cells|
|Positive IP detected in||mouse kidney tissue|
|Positive IHC detected in||human heart tissue, human spleen tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14276-1-AP targets CNOT1 in WB, IP, IHC, IF, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CNOT1 fusion protein Ag5623|
|Full Name||CCR4-NOT transcription complex, subunit 1|
|Calculated molecular weight||267 kDa|
|Observed molecular weight||240-250 kDa, 174 kDa|
|GenBank accession number||BC040523|
|Gene ID (NCBI)||23019|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CNOT1 is a component of CCR4-NOT protein complex, which is a regulator of RNA polymerase II transcription, acts as a transcription repressor. CCR4-NOT complex could participate in transcription as well as mRNA degradation. It's highly expressed in brain, heart, thymus, but weak in skeletal muscle and colon. CNOT1 undergoes alternative splicing to produce four isoforms.This is a rabbit polyclonal antibody raised against part chain of C-terminal CNOT1 of human origin. CNOT1 exsits as many isoforms and molecular weight of isoforms are 267, 241 and 173 kDa.
DND1 maintains germline stem cells via recruitment of the CCR4-NOT complex to target mRNAs.
Roquin promotes constitutive mRNA decay via a conserved class of stem-loop recognition motifs.
The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity.
Dynamic Recruitment of Single RNAs to Processing Bodies Depends on RNA Functionality.
Endoribonucleolytic Cleavage of m6A-Containing RNAs by RNase P/MRP Complex.
Multivalent Proteins Rapidly and Reversibly Phase-Separate upon Osmotic Cell Volume Change.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
aditya (Verified Customer) (10-25-2022)
This is brilliant. We use this for cnot1 pull downs and then use a seperate cnot1 antibody to image on the western. Great for identifying interacting partners.
Tobias (Verified Customer) (10-25-2022)
Parisa (Verified Customer) (10-17-2022)
good quality, high sensitivity
Will (Verified Customer) (01-31-2019)
good antibody, would use again