EIF2C1-Specific Polyclonal antibody

EIF2C1-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 19690-1-AP

Synonyms

AGO1, Argonaute1, eIF 2C 1, EIF2C, eIF2C 1, EIF2C1, EIF2C1-Specific, GERP95, hAgo1, Protein argonaute 1, Q99

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Validation Data Gallery

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  WB analysis of HeLa using 19690-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 19690-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 19690-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 19690-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 19690-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 19690-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 19690-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 19690-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 19690-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary tumor using 19690-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 19690-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 19690-1-AP

  Immunofluorescent analysis of HepG2 cells using 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  IF Staining of HepG2 using 19690-1-AP

  Immunofluorescent analysis of HepG2 cells using 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, NIH/3T3 cells
• Positive IHC detected in: human cervical cancer tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 2 publications below

Product Information

19690-1-AP targets EIF2C1-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: eukaryotic translation initiation factor 2C, 1
• Calculated Molecular Weight: 97 kDa
• Observed Molecular Weight: 97 kDa
• GenBank Accession Number: NM_012199
• Gene Symbol: EIF2C1
• Gene ID (NCBI): 26523
• RRID: AB_10863657
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EIF2C1, also named as AGO1, belongs to the argonaute family and Ago subfamily. EIF2C1 is required for RNA-mediated gene silencing (RNAi). It binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. EIF2C1 may also be required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs). The antibody is specific to EIF2C1.

Publications

Species	Application	Title
human	IF	Front Mol Neurosci; miRNA Enriched in Human Neuroblast Nuclei Bind the MAZ Transcription Factor and Their Precursors Contain the MAZ Consensus Motif.; Authors - Belinda J Goldie
human	WB	Mol Ther Oncolytics; Hsa_circ_0001666 promotes non-small cell lung cancer migration and invasion through miR-1184/miR-548I/AGO1 axis.; Authors - Xueting Wang
mouse	IF	bioRxiv; MicroRNAs are necessary for the emergence of Purkinje cell identity; Authors - Norjin Zolboot

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Roy (Verified Customer) (06-15-2024)

Unfortunately it did detect both Ago1 and Ago2 (validation from siRNA experiments)

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa