Anticorps Polyclonal de lapin anti-DCC-Specific
DCC-Specific Polyclonal Antibody for WB, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris
Applications
WB, IF, IHC, ELISA
Conjugaison
Non conjugué
N° de cat : 19123-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | tissu cérébral de souris |
Dilution recommandée
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
WB | See 2 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
Informations sur le produit
19123-1-AP cible DCC-Specific dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
Réactivité | Humain, rat, souris |
Réactivité citée | rat, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | Peptide |
Nom complet | deleted in colorectal carcinoma |
Masse moléculaire calculée | 1447 aa, 158 kDa |
Poids moléculaire observé | 180 kDa |
Numéro d’acquisition GenBank | NM_005215 |
Symbole du gène | DCC |
Identification du gène (NCBI) | 1630 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
DCC, also named as IGDCC1, belongs to the immunoglobulin superfamily and DCC family. DCC is a receptor for netrin required for axon guidance. DCC mediates axon attraction of neuronal growth cones in the developing nervous system upon ligand binding. Its association with UNC5 proteins may trigger signaling for axon repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. DCC gene is a tumor suppressor gene. This antibody raised against a synthesized polypeptide of human DCC protein recognizes endogenous DCC with an apparent molecular weight of 180 kDa. As the predicted molecular mass of DCC is 158 kDa, the slower mobility is likely attributable to post-translational modification, perhaps by glycosylation at potential N-linked glycosylation sites (PMID: 7926722; 8044801).
Protocole
Product Specific Protocols | |
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WB protocol for DCC-Specific antibody 19123-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Sci Rep The expression of Netrin-1 in the MIA-induced osteoarthritic temporomandibular joint in mice. | ||
Front Pharmacol Cinnamaldehyde attenuates streptozocin-induced diabetic osteoporosis in a rat model by modulating netrin-1/DCC-UNC5B signal transduction |