UNC5A Polyclonal antibody

UNC5A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 22068-1-AP

Synonyms

KIAA1976, Netrin receptor UNC5A, Protein unc 5 homolog 1, Protein unc 5 homolog A, unc 5 homolog A (C. elegans), UNC5A, UNC5H1

---

Validation Data Gallery

• 

  WB analysis of HeLa using 22068-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22068-1-AP (UNC5A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 22068-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 22068-1-AP (UNC5A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 22068-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 22068-1-AP (UNC5A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 22068-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22068-1-AP (UNC5A Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 22068-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22068-1-AP (UNC5A Antibody) at dilution of 1:200 (under 40x lens).

---

Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, PC-3 cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

The immunogen of 22068-1-AP is UNC5A Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UNC5A fusion protein Ag17155
• Full Name: unc-5 homolog A (C. elegans)
• Calculated Molecular Weight: 842 aa, 93 kDa
• Observed Molecular Weight: 88-100 kDa
• GenBank Accession Number: BC157824
• Gene Symbol: UNC5A
• Gene ID (NCBI): 90249
• RRID: AB_11182161
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UNC5A, also named as KIAA1976 and UNC5H1, belongs to the unc-5 family. It is a receptor for netrin required for axon guidance. It mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand.

Publications

Species	Application	Title
mouse	IF	Mol Psychiatry; YTHDF2 in dentate gyrus is the m6A reader mediating m6A modification in hippocampus-dependent learning and memory; Authors - Mengru Zhuang
human	IHC	Int J Clin Exp Pathol; The association of UNC5A expression with the clinicopathologic features and prognosis of radiotherapy in patients with non-small-cell lung cancer.; Authors - Silu Ding
human	WB	Cell Commun Signal; Netrin-1 induces the anti-apoptotic and pro-survival effects of B-ALL cells through the Unc5b-MAPK axis; Authors - Lan Huang
human,mouse	IHC,IF	Cancers (Basel); Upregulation of Cell Surface Glycoproteins in Correlation with KSHV LANA in the Kaposi Sarcoma Tumor Microenvironment; Authors - Sara R Privatt