CBLN4 Polyclonal antibody

CBLN4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20559-1-AP

Synonyms

CBLN4, CBLNL1, Cerebellin 4, cerebellin 4 precursor, Cerebellin like glycoprotein 1

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Validation Data Gallery

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  WB analysis of mouse cerebellum using 20559-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 20559-1-AP (CBLN4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20559-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20559-1-AP (CBLN4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 20559-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20559-1-AP (CBLN4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 20559-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20559-1-AP (CBLN4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cerebellum using 20559-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum using 20559-1-AP (CBLN4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cerebellum using 20559-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum using 20559-1-AP (CBLN4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 20559-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20559-1-AP (CBLN4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 20559-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20559-1-AP (CBLN4 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse cerebellum tissue, mouse brain tissue, mouse kidney tissue
• Positive IHC detected in: human cerebellum tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20559-1-AP targets CBLN4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: cerebellin 4 precursor
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: NM_080617
• Gene Symbol: CBLN4
• Gene ID (NCBI): 140689
• RRID: AB_2878701
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CBLN4 may be involved in synaptic functions in the CNS. CBLN4 can enable ER export and secretion of CBLN3. The antibody has no cross reaction to CBLN1, 2, or 3.