UNC5A-Specific Polyclonal antibody

UNC5A-Specific Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20239-1-AP

Synonyms

UNC5H1, UNC5A, Protein unc-5 homolog A, Protein unc-5 homolog 1, Netrin receptor UNC5A

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Validation Data Gallery

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  WB analysis of mouse brain using 20239-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20239-1-AP (UNC5A-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20239-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20239-1-AP (UNC5A-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20239-1-AP targets UNC5A-Specific in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: unc-5 homolog A (C. elegans)
• Calculated Molecular Weight: 93 kDa
• Observed Molecular Weight: 103 kDa
• GenBank Accession Number: NM_133369
• Gene Symbol: UNC5A
• Gene ID (NCBI): 90249
• RRID: AB_10695050
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UNC5A, also named as KIAA1976 and UNC5H1, belongs to the unc-5 family. It is a receptor for netrin required for axon guidance. It mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. The antibody is specific to UNC5A. It has no cross reaction to UNC5B, UNC5C and UNC5D.

Publications

Species	Application	Title
human		Sci Adv; The FLRT3-UNC5B checkpoint pathway inhibits T cell-based cancer immunotherapies; Authors - Kushal Prajapati