Anticorps Polyclonal de lapin anti-CXCL12/SDF-1

• Phare
• Validé par KD/KO

CXCL12/SDF-1 Polyclonal Antibody for IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, FC (Intra), ELISA, IF

Conjugaison

Non conjugué

Publications

52

Avis (1)

N° de cat : 17402-1-AP

Synonymes

CXCL12, C-X-C motif chemokine 12, PBSF, IRH, hSDF-1

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Galerie de données de validation

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  IHC staining of human liver cancer using 17402-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17402-1-AP (CXCL12/SDF-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 17402-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17402-1-AP (CXCL12/SDF-1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of Jurkat using 17402-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human CXCL12/SDF-1 (17402-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules Jurkat,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 24 publications below
• IHC: See 17 publications below
• IF: See 18 publications below

Informations sur le produit

17402-1-AP cible CXCL12/SDF-1 dans les applications de WB, IHC, FC (Intra), ELISA, IF et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CXCL12/SDF-1 Protéine recombinante Ag11379
• Nom complet: chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1)
• Masse moléculaire calculée: 89 aa, 10 kDa
• Numéro d’acquisition GenBank: BC039893
• Symbole du gène: CXCL12/SDF-1
• Identification du gène (NCBI): 6387
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

In adulthood, CXCL12 plays an important role in angiogenesis by recruiting endothelial progenitor cells (EPCs) from the bone marrow through a CXCR4 dependent mechanism (PMID: 17878755). It is this function of CXCL12 that makes it a very important factor in carcinogenesis and the neovascularisation linked to tumour progression (PMID: 16943240). CXCL12 also has a role in tumor metastasis where cancer cells that express the receptor CXCR4 are attracted to metastasis target tissues that release the ligand, CXCL12 (PMID: 11242036 ). In breast cancer, however, increased expression of CXCL12 determines a reduced risk of distant metastasis (PMID: 19646861; 17724466 ). This antibody can detect all the isoforms of CXCL12/SDF1.

Publications

Species	Application	Title
	IF	Nature; Tumoural activation of TLR3-SLIT2 axis in endothelium drives metastasis.; Authors - Bernardo Tavora
rat	WB	Acta Biomater; Sodium alginate/collagen/stromal cell-derived factor-1 neural scaffold loaded with BMSCs promotes neurological function recovery after traumatic brain injury.; Authors - Shanshan Ma
mouse	WB	Biomed Pharmacother; Angelica sinensis polysaccharides ameliorated 5-Fluorouracil-induced damage of early B cell progenitors by alleviating oxidative stress of IL-7 producing mesenchymal stem and progenitor cells; Authors - Hanxianzhi Xiao
human		Mol Oncol; Growth arrest-specific protein 2 (GAS2) interacts with CXCR4 to promote T-cell leukemogenesis partially via c-MYC; Authors - Yun Zhao
human	WB	J Nanobiotechnology; Magnetic targeting enhances the cutaneous wound healing effects of human mesenchymal stem cell-derived iron oxide exosomes.; Authors - Xiuying Li
human	WB	Fertil Steril; Protein kinase CK2 participates in estrogen-mediated endothelial progenitor cell homing to endometriotic lesions through stromal cells in a stromal cell-derived factor-1- CXCR4-dependent manner.; Authors - Rong Zhao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Yohanes (Verified Customer) (09-16-2022)

Good specificity antibody for immunofluoresence experiment.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse brain endothelial cell

CXCL12/SDF-1 Antibody Western Blot,Immunofluorescence validation (1:1000 dilution) in Mouse brain endothelial cell (Cat no:17402-1-AP)

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