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VAV1 Polyclonal antibody
VAV1 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 16364-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, K-562 cells, HL-60 cells |
Positive IP detected in | K-562 cells |
Positive IHC detected in | human breast cancer tissue, human brain tissue, human kidney tissue, human liver tissue, human heart tissue, human spleen tissue, human lung tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 2 publications below |
WB | See 6 publications below |
IHC | See 1 publications below |
IF | See 3 publications below |
Product Information
16364-1-AP targets VAV1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, rat, gecko |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | VAV1 fusion protein Ag9049 |
Full Name | vav 1 guanine nucleotide exchange factor |
Calculated Molecular Weight | 845 aa, 98 kDa |
Observed Molecular Weight | 98 kDa |
GenBank Accession Number | BC013361 |
Gene Symbol | VAV1 |
Gene ID (NCBI) | 7409 |
RRID | AB_2213571 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for VAV1 antibody 16364-1-AP | Download protocol |
IHC protocol for VAV1 antibody 16364-1-AP | Download protocol |
IP protocol for VAV1 antibody 16364-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Biol Chem Adult astrocytes from reptiles are resistant to proinflammatory activation via sustaining Vav1 expression.
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Biochem Biophys Res Commun Vav1 expression is increased in esophageal squamous cell carcinoma and indicates poor prognosis. | ||
Oncotarget Multiple regulatory aspects of histone methyltransferase EZH2 in Pb-induced neurotoxicity. | ||
Clin Sci (Lond) Novel role of Vav1-Rac1 pathway in actin cytoskeleton regulation in Interleukin-13-induced minimal change-like nephropathy.
| ||
Cancer Cell Int STING pathway contributes to the prognosis of hepatocellular carcinoma and identification of prognostic gene signatures correlated to tumor microenvironment | ||
Neural Regen Res Vav1 promotes inflammation and neuronal apoptosis in cerebral ischemia/reperfusion injury by upregulating microglial and NLRP3 inflammasome activation |