• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-Cathepsin D

Cathepsin D Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 21327-1-AP

Synonymes

cathepsin D, CLN10, CPSD, CTSD



Applications testées

Résultats positifs en WBcellules A375, cellules HepG2, cellules HuH-7, cellules MCF-7
Résultats positifs en IHCtissu de cirrhose hépatique humain,
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochimie (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

21327-1-AP cible Cathepsin D dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéerat, bovin, Humain, porc, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Cathepsin D Protéine recombinante Ag15254
Nom complet cathepsin D
Masse moléculaire calculée 412 aa, 45 kDa
Poids moléculaire observé 32 kDa, 48 kDa, 52 kDa
Numéro d’acquisition GenBankBC016320
Symbole du gène Cathepsin D
Identification du gène (NCBI) 1509
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CTSD (Cathepsin D) also named CPSD, belongs to the peptidase A1 family. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Human CTSD is synthesized as a 52-kDa precursor that is converted into an active 48-kDa single-chain intermediate in the endosomes, and then into a fully active mature form, composed of a 34-kDa heavy chain and a 14-kDa light chain, in the lysosomes. It is a lysosomal acid protease found in neutrophils and monocytes and involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. (PMID: 27114232, PMID: 30717773, PMID: 30051532)

Protocole

Product Specific Protocols
WB protocol for Cathepsin D antibody 21327-1-APDownload protocol
IHC protocol for Cathepsin D antibody 21327-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Acta Neuropathol

Wolframin is a novel regulator of tau pathology and neurodegeneration.

Authors - Shuo Chen
mouseWB

Mol Cell

Itaconate is a lysosomal inducer that promotes antibacterial innate immunity.

Authors - Zhenxing Zhang
mouseWB

Acta Pharm Sin B

Nuciferine protects against high-fat diet-induced hepatic steatosis and insulin resistance via activating TFEB-mediated autophagy-lysosomal pathway.

Authors - Xiliang Du
mouseWB,IF

Redox Biol

Cyclic helix B peptide alleviates proinflammatory cell death and improves functional recovery after traumatic spinal cord injury

Authors - Yu Xu
human,mouseWB,IF

Autophagy

Restoration of CTSD (cathepsin D) and lysosomal function in stroke is neuroprotective.

Authors - M Iqbal Hossain
  • KD Validated
mouseWB

Cell Death Differ

Programmed cell death 4 modulates lysosomal function by inhibiting TFEB translation.

Authors - Xiaotong Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Chen (Verified Customer) (06-01-2021)

Very good to detect cathepsin D in postmortem human samples with Western blotting

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: postmortem human brain lysate
Cathepsin D Antibody Western Blot validation (1:3000 dilution) in postmortem human brain lysate (Cat no:21327-1-AP)
FH

Efil (Verified Customer) (05-29-2019)

cells were fixed in cold MeOH for 8 minutes

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: human fibroblasts
Cathepsin D Antibody Immunofluorescence, validation (1:100 dilution) in human fibroblasts (Cat no:21327-1-AP)