Cathepsin A Polyclonal antibody

Cathepsin A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15020-1-AP

Synonyms

Carboxypeptidase C, Carboxypeptidase L, Cathepsin A, CTSA, GLB2, GSL, Lysosomal protective protein, NGBE, PPCA, PPGB, Protective protein cathepsin A



Tested Applications

Positive WB detected inA549 cells, Sp2/0 cells, PC-3 cells, DU 145 cells
Positive IHC detected inhuman liver tissue, human breast cancer tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:750-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15020-1-AP targets Cathepsin A in WB, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cathepsin A fusion protein Ag7073
Full Name cathepsin A
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 54-60, 32, 20 kDa
GenBank Accession NumberBC000597
Gene Symbol Cathepsin A
Gene ID (NCBI) 5476
RRIDAB_10696309
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTSA is also named as PPGB(protective protein for beta-galactosidase), PPCA(protective protein cathepsin A), cathepsin A, carboxypeptidase C, carboxypeptidase L and belongs to the peptidase S10 family. It is a ubiquitously expressed multifunctional enzyme, with deamidase, esterase, and carboxypeptidase activities and a preference for substrates with hydrophobic amino acid residues at the P1-prime position. It can be cleaved into the following 2 chains and defects in CTSA are the cause of galactosialidosis (GSL). This protein has 2 glycosylation sites. CTSA is synthesized as a 54-kDa precursor protein and composed of 32- and 20-kDa subunits linked together by disulfide bonds(PMID: 16461364,19574551).

Protocols

Product Specific Protocols
WB protocol for Cathepsin A antibody 15020-1-APDownload protocol
IHC protocol for Cathepsin A antibody 15020-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Hepatol

SNX10 mediates alcohol-induced liver injury and steatosis via regulating chaperone-mediated autophagy activation.

Authors - Yan You
humanIHC

Am J Cancer Res

High cathepsin A protein expression predicts poor prognosis and tumor recurrence of hepatocellular carcinoma patients after curative hepatectomy

Authors - Laibang Luo
mouseWB

Mol Carcinog

Resistance to abemaciclib is associated with increased metastatic potential and lysosomal protein deregulation in breast cancer cells

Authors - Erin R Scheidemann
humanIHC

Sci Rep

Prognostic significance and oncogene function of cathepsin A in hepatocellular carcinoma.

Authors - Huaxiang Wang
mouseWB

Front Cell Neurosci

Neu1 Is Released From Activated Microglia, Stimulating Microglial Phagocytosis and Sensitizing Neurons to Glutamate.

Authors - David H Allendorf
mouseFC

Nature

Anti-tumour immunity controlled through mRNA m6A methylation and YTHDF1 in dendritic cells.

Authors - Dali Han