STK11/LKB1 Polyclonal antibody

• Featured Product
• KD/KO Validated

STK11/LKB1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

51

Reviews (1)

Cat no : 10746-1-AP

Synonyms

LKB1, STK11, STK11/ LKB1, Renal carcinoma antigen NY-REN-19, PJS

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Validation Data Gallery

• 

  WB analysis of HepG2 using 10746-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10746-1-AP (LKB1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 10746-1-AP

  IP result of anti-STK11/LKB1 (IP:10746-1-AP, 4ug; Detection:10746-1-AP 1:500) with HEK-293 cells lysate 2000ug.

• 

  IHC staining of human lung cancer using 10746-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10746-1-AP (STK11/LKB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10746-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10746-1-AP (STK11/LKB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10746-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10746-1-AP (STK11/LKB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 10746-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10746-1-AP (STK11/LKB1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 10746-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10746-1-AP (STK11/LKB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human lung cancer tissue, human testis tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 45 publications below
• IHC: See 3 publications below
• IF: See 5 publications below
• IP: See 6 publications below
• CoIP: See 4 publications below

Product Information

10746-1-AP targets STK11/LKB1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STK11/LKB1 fusion protein Ag1048
• Full Name: serine/threonine kinase 11
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC007981
• Gene Symbol: LKB1
• Gene ID (NCBI): 6794
• RRID: AB_2271311
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STK11(serine/threonine-protein kinase 11) is also named as LKB1, PJS, and belongs to the protein kinase superfamily. It controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. The tumour suppressor protein LKB1 is a serine/threonine kinase that has been causally linked to Peutz-Jeghers syndrome (PJS). Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) and defects in STK11 have been associated with testicular germ cell tumor (TGCT) and some sporadic cancers, especially lung cancers. STK11 has 2 isoforms with MW of 49 kDa and 45 kDa, and can be detected as 50-54 kDa after posttranslational modification.

Publications

Species	Application	Title
human	WB	Cell Metab; CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress.; Authors - Qidong Li ; KD Validated
human	WB	Nat Commun; Cordycepin prevents radiation ulcer by inhibiting cell senescence via NRF2 and AMPK in rodents.; Authors - Ziwen Wang
mouse	WB	EBioMedicine; Exogenous NADPH exerts a positive inotropic effect and enhances energy metabolism via SIRT3 in pathological cardiac hypertrophy and heart failure; Authors - Ke Qian
human	WB	EMBO J; CARS senses cysteine deprivation to activate AMPK for cell survival.; Authors - Mengqiu Yuan ; KD Validated
mouse	WB	Kidney Int; Rho-associated, coiled-coil-containing protein kinase 1 regulates development of diabetic kidney disease via modulation of fatty acid metabolism.; Authors - Yosuke Nagai
rat	CoIP	Aging Cell; Antagonizing peroxisome proliferator-activated receptor γ facilitates M1-to-M2 shift of microglia by enhancing autophagy via the LKB1-AMPK signaling pathway.; Authors - Juan Ji

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Bogdan (Verified Customer) (11-29-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: nerve

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