Anticorps Polyclonal de lapin anti-COMMD1

• Phare
• Validé par KD/KO

COMMD1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

22

Avis (1)

N° de cat : 11938-1-AP

Synonymes

C2orf5, COMMD1, MURR1, Protein Murr1

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Galerie de données de validation

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  WB analysis using 11938-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11938-1-AP (COMMD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 11938-1-AP

  IP result of anti-COMMD1 (IP:11938-1-AP, 3ug; Detection:11938-1-AP 1:500) with mouse brain tissue lysate 5200ug.

• 

  IHC staining of human liver cancer using 11938-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11938-1-AP (COMMD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11938-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11938-1-AP (COMMD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11938-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using COMMD1 antibody (11938-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 19 publications below
• IHC: See 4 publications below
• IF: See 5 publications below
• CoIP: See 2 publications below

Informations sur le produit

11938-1-AP cible COMMD1 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: COMMD1 Protéine recombinante Ag2535
• Nom complet: copper metabolism (Murr1) domain containing 1
• Masse moléculaire calculée: 190 aa, 21 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC022046
• Symbole du gène: COMMD1
• Identification du gène (NCBI): 150684
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

COMMD1(COMM domain-containing protein 1), also named MURR1, is implicated in copper homeostasis by binding one copper ion per monomer. COMMD1 was reported to accelerate the ubiquitination and degradation of NF-kappa-B subunits, its deficiency exerts enhanced NF-kappa-B mediated celluar response. Ubiquitinated by XIAP (X-linked inhibitor of apoptosis), COMMD1 undergoes proteasomal degradation. SOD1 activity can be downregulated by COMMD1.

Publications

Species	Application	Title
human,mouse	WB,IF	Circ Res; COMMD Family Regulates Plasma LDL Levels and Attenuates Atherosclerosis Through Stabilizing the CCC Complex in Endosomal LDLR Trafficking.; Authors - Alina Fedoseienko ; KD Validated
human	WB	Nat Commun; A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry.; Authors - Yunkai Zhu
canine	WB,IF	Nat Commun; CCC- and WASH-mediated endosomal sorting of LDLR is required for normal clearance of circulating LDL.; Authors - Paulina Bartuzi ; KO Validated
human		J Cell Biol; HRS-WASH axis governs actin-mediated endosomal recycling and cell invasion.; Authors - Ewan MacDonald
human	WB,IF,CoIP	Cell Rep; COMMD5/HCaRG Hooks Endosomes on Cytoskeleton and Coordinates EGFR Trafficking.; Authors - Carole G Campion ; KD Validated
human	WB	Theranostics; SPTBN1 inhibits inflammatory responses and hepatocarcinogenesis via the stabilization of SOCS1 and downregulation of p65 in hepatocellular carcinoma.; Authors - Ling Lin

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Emilia (Verified Customer) (11-30-2018)

works well for WB

• Applications: Western Blot,
• Primary Antibody Dilution: 1;1000
• Cell Tissue Type: 293, Cos1

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