ATP7B-Specific Polyclonal antibody

ATP7B-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 19786-1-AP

Synonyms

ATP7B, Wilson disease-associated protein, WC1, EC:7.2.2.8, Copper-transporting ATPase 2

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Validation Data Gallery

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  WB analysis using 19786-1-AP

  Mouse liver and rat liver tissues were subjected to SDS PAGE followed by western blot with 19786-1-AP (ATP7B-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 19786-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19786-1-AP (ATP7B-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19786-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19786-1-AP (ATP7B-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19786-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19786-1-AP (ATP7B-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 19786-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19786-1-AP (ATP7B-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 19786-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19786-1-AP (ATP7B-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19786-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19786-1-AP (ATP7B-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 19786-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ATP7B-Specific antibody (19786-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue
• Positive IHC detected in: human colon cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

19786-1-AP targets ATP7B-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: ATPase, Cu++ transporting, beta polypeptide
• Calculated Molecular Weight: 157 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: NM_000053
• Gene Symbol: ATP7B
• Gene ID (NCBI): 540
• RRID: AB_2878607
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP7B, also named as PWD, WC1 and WND, belongs to the cation transport ATPase (P-type) family and Type IB subfamily. ATP7B is involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile. ATP7B catalyzes the reaction: ATP + H2O + Cu2+(In) = ADP + phosphate + Cu2+(Out). Defects in ATP7B are the cause of Wilson disease (WD). This antibody is specific to ATP7B.

Publications

Species	Application	Title
mouse	WB	Adv Healthc Mater; Copper-Based Composites Nanoparticles Improve Triple-Negative Breast Cancer Treatment with Induction of Apoptosis-Cuproptosis and Immune Activation; Authors - Ning Wang
human	IHC	Comput Struct Biotechnol J; Impact of Cuproptosis-related markers on clinical status, tumor immune microenvironment and immunotherapy in colorectal cancer: A multi-omic analysis; Authors - Yanfei Shao
human	WB,IHC	Gene; Integrative analysis of the cuproptosis-related gene ATP7B in the prognosis and immune infiltration of IDH1 wild-type glioma; Authors - Kun Zhang
human	IF	Medicina (Kaunas); Curcumin Effect on Copper Transport in HepG2 Cells.; Authors - Anita Berzina
rat	WB	Ann Med; Dexmedetomidine enables copper homeostasis in cerebral ischemia/reperfusion via ferredoxin 1; Authors - Qingduo Guo
mouse	WB	iScience; Hepatic depletion of nucleolar protein mDEF causes excessive mitochondrial copper accumulation associated with p53 and NRF1 activation; Authors - Jinsong Wei