RAB7A Polyclonal antibody

• Featured Product
• KD/KO Validated

RAB7A Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

29

Reviews (4)

Cat no : 55469-1-AP

Synonyms

Endosome Marker, PRO2706, RAB7, RAB7A, Ras related protein Rab 7a

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Validation Data Gallery

• 

  WB analysis using 55469-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55469-1-AP (RAB7A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 55469-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 55469-1-AP (RAB7A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 55469-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55469-1-AP (RAB7A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 55469-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 55469-1-AP (RAB7A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SH-SY5Y using 55469-1-AP

  IP result of anti-RAB7A (IP:55469-1-AP, 4ug; Detection:55469-1-AP 1:1000) with SH-SY5Y cells lysate 3360 ug.

• 

  IHC staining of human kidney using 55469-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55469-1-AP (RAB7A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 55469-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55469-1-AP (RAB7A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 55469-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 55469-1-AP (RAB7A Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 55469-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 55469-1-AP (RAB7A Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach using 55469-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 55469-1-AP (RAB7A Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach using 55469-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 55469-1-AP (RAB7A Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: L-929 cells, SH-SY5Y cells, A431 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue, C6 cells, RAW 264.7 cells
• Positive IP detected in: SH-SY5Y cells
• Positive IHC detected in: human kidney tissue, human skeletal muscle tissue, human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 4 publications below
• WB: See 19 publications below
• IHC: See 1 publications below
• IF: See 10 publications below

Product Information

55469-1-AP targets RAB7A in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey, pig, Zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: RAB7A, member RAS oncogene family
• Calculated molecular weight: 23 kDa
• Observed molecular weight: 23 kDa
• GenBank accession number: NM_004637
• Gene symbol: RAB7A
• Gene ID (NCBI): 7879
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage.

Background Information

RAB7A, also named as RAB7, belongs to the small GTPase superfamily and Rab family. It is involved in late endocytic transport. RAB7A contributes to the maturation of phagosomes (acidification). Defects in RAB7A are the cause of Charcot-Marie-Tooth disease type 2B (CMT2B). This antibody is specific to RAB7A.

Publications

Species	Application	Title
mouse	IF	Aging (Albany NY); Cytoplasmic PCNA is located in the actin belt and involved in osteoclast differentiation.; Authors - Donge Tang
human	IF	Cell Death Dis; FV-429 induces autophagy blockage and lysosome-dependent cell death of T-cell malignancies via lysosomal dysregulation.; Authors - Po Hu
human	IF,WB	Cell Death Dis; TRIM39 deficiency inhibits tumor progression and autophagic flux in colorectal cancer via suppressing the activity of Rab7.; Authors - Jia Hu ; KD Validated
human	WB, IF	Signal Transduct Target Ther; Nlp promotes autophagy through facilitating the interaction of Rab7 and FYCO1.; Authors - Wenchang Xiao
human	IHC	Open Med (Wars); Downregulation of RAB7 and Caveolin-1 increases MMP-2 activity in renal tubular epithelial cells under hypoxic conditions.; Authors - Wenmin Yu
human	WB	Int J Mol Sci; Reactive Oxygen Species Mediate 6c-Induced Mitochondrial and Lysosomal Dysfunction, Autophagic Cell Death, and DNA Damage in Hepatocellular Carcinoma.; Authors - Senzhen Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Azita (Verified Customer) (06-17-2021)

Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by RAB7A Polyclonal antibody at dilution of 1:50 showed strong labelling.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: NSC-34 cells

FH

Laura (Verified Customer) (01-15-2020)

Works well for Western Blot at 1:1000 dilution.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

FH

Aamir (Verified Customer) (01-08-2020)

WB at 1 in 500IF at 1 in 250

• Applications: Western Blot, Immunohistochemistry,
• Cell Tissue Type: HEKs

FH

Benjamin (Verified Customer) (12-10-2019)

Has worked well multiple times with dilution of 1:1000 with the RAB7A antibody. I always incubate at 4 degrees overnight in 3% milk TBST solution.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293 Lysate

RAB7A Antibody Western Blot, validation (1:1000 dilution) in HEK293 Lysate (Cat no:55469-1-AP)

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