- Featured Product
- KD/KO Validated
RAB7A Polyclonal antibody
RAB7A Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (3)
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 55469-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | L-929 cells, SH-SY5Y cells, A431 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue, C6 cells, RAW 264.7 cells |
Positive IP detected in | SH-SY5Y cells |
Positive IHC detected in | human kidney tissue, human skeletal muscle tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 4 publications below |
WB | See 19 publications below |
IHC | See 1 publications below |
IF | See 10 publications below |
Product Information
55469-1-AP targets RAB7A in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, monkey, pig, Zebrafish |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | RAB7A, member RAS oncogene family |
Calculated molecular weight | 23 kDa |
Observed molecular weight | 23 kDa |
GenBank accession number | NM_004637 |
Gene symbol | RAB7A |
Gene ID (NCBI) | 7879 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
RAB7A, also named as RAB7, belongs to the small GTPase superfamily and Rab family. It is involved in late endocytic transport. RAB7A contributes to the maturation of phagosomes (acidification). Defects in RAB7A are the cause of Charcot-Marie-Tooth disease type 2B (CMT2B). This antibody is specific to RAB7A.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for RAB7A antibody 55469-1-AP | Download protocol |
IHC protocol for RAB7A antibody 55469-1-AP | Download protocol |
IP protocol for RAB7A antibody 55469-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Aging (Albany NY) Cytoplasmic PCNA is located in the actin belt and involved in osteoclast differentiation. | ||
Cell Death Dis FV-429 induces autophagy blockage and lysosome-dependent cell death of T-cell malignancies via lysosomal dysregulation. | ||
Cell Death Dis TRIM39 deficiency inhibits tumor progression and autophagic flux in colorectal cancer via suppressing the activity of Rab7.
| ||
Signal Transduct Target Ther Nlp promotes autophagy through facilitating the interaction of Rab7 and FYCO1. | ||
Open Med (Wars) Downregulation of RAB7 and Caveolin-1 increases MMP-2 activity in renal tubular epithelial cells under hypoxic conditions. | ||
Int J Mol Sci Reactive Oxygen Species Mediate 6c-Induced Mitochondrial and Lysosomal Dysfunction, Autophagic Cell Death, and DNA Damage in Hepatocellular Carcinoma. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Azita (Verified Customer) (06-17-2021) | Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by RAB7A Polyclonal antibody at dilution of 1:50 showed strong labelling.
|
FH Laura (Verified Customer) (01-15-2020) | Works well for Western Blot at 1:1000 dilution.
|
FH Aamir (Verified Customer) (01-08-2020) | WB at 1 in 500IF at 1 in 250
|
FH Benjamin (Verified Customer) (12-10-2019) | Has worked well multiple times with dilution of 1:1000 with the RAB7A antibody. I always incubate at 4 degrees overnight in 3% milk TBST solution.
![]() |