Validation Data Gallery
|Positive WB detected in||L-929 cells, SH-SY5Y cells, A431 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue, C6 cells, RAW 264.7 cells|
|Positive IP detected in||SH-SY5Y cells|
|Positive IHC detected in||human kidney tissue, human skeletal muscle tissue, human stomach tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
55469-1-AP targets RAB7A in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, monkey, pig, Zebrafish|
|Host / Isotype||Rabbit / IgG|
|Full Name||RAB7A, member RAS oncogene family|
|Calculated molecular weight||23 kDa|
|Observed molecular weight||23 kDa|
|GenBank accession number||NM_004637|
|Gene ID (NCBI)||7879|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
RAB7A, also named as RAB7, belongs to the small GTPase superfamily and Rab family. It is involved in late endocytic transport. RAB7A contributes to the maturation of phagosomes (acidification). Defects in RAB7A are the cause of Charcot-Marie-Tooth disease type 2B (CMT2B). This antibody is specific to RAB7A.
Aging (Albany NY)
Cytoplasmic PCNA is located in the actin belt and involved in osteoclast differentiation.
Cell Death Dis
FV-429 induces autophagy blockage and lysosome-dependent cell death of T-cell malignancies via lysosomal dysregulation.
Cell Death Dis
TRIM39 deficiency inhibits tumor progression and autophagic flux in colorectal cancer via suppressing the activity of Rab7.
Signal Transduct Target Ther
Nlp promotes autophagy through facilitating the interaction of Rab7 and FYCO1.
Open Med (Wars)
Downregulation of RAB7 and Caveolin-1 increases MMP-2 activity in renal tubular epithelial cells under hypoxic conditions.
Int J Mol Sci
Reactive Oxygen Species Mediate 6c-Induced Mitochondrial and Lysosomal Dysfunction, Autophagic Cell Death, and DNA Damage in Hepatocellular Carcinoma.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Azita (Verified Customer) (06-17-2021)
Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by RAB7A Polyclonal antibody at dilution of 1:50 showed strong labelling.
Laura (Verified Customer) (01-15-2020)
Works well for Western Blot at 1:1000 dilution.
Aamir (Verified Customer) (01-08-2020)
WB at 1 in 500IF at 1 in 250
Benjamin (Verified Customer) (12-10-2019)
Has worked well multiple times with dilution of 1:1000 with the RAB7A antibody. I always incubate at 4 degrees overnight in 3% milk TBST solution.