Tested Applications
| Positive WB detected in | rat brain tissue, A431 cells, L-929 cells, NIH/3T3 cells, SH-SY5Y cells, RAW 264.7 cells, C6 cells, mouse brain tissue |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human kidney tissue, human skeletal muscle tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse spinal cord tissue |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55469-1-AP targets RAB7A in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, monkey, zebrafish, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | RAB7A, member RAS oncogene family |
| Calculated Molecular Weight | 23 kDa |
| Observed Molecular Weight | 23 kDa |
| GenBank Accession Number | NM_004637 |
| Gene Symbol | RAB7A |
| Gene ID (NCBI) | 7879 |
| RRID | AB_11182831 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P51149 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RAB7A, also named as RAB7, belongs to the small GTPase superfamily and Rab family. It is involved in late endocytic transport. RAB7A contributes to the maturation of phagosomes (acidification). Defects in RAB7A are the cause of Charcot-Marie-Tooth disease type 2B (CMT2B). This antibody is specific to RAB7A.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for RAB7A antibody 55469-1-AP | Download protocol |
| IF protocol for RAB7A antibody 55469-1-AP | Download protocol |
| IHC protocol for RAB7A antibody 55469-1-AP | Download protocol |
| IP protocol for RAB7A antibody 55469-1-AP | Download protocol |
| WB protocol for RAB7A antibody 55469-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RAB7A Polyclonal Antibody (Cat# 55469-1-AP) has 112 citations across 60+ journals including Nature Communications, Autophagy, Cell Research, Cell Death & Disease, Advanced Science, PLoS Pathogens, Journal of Virology, and International Journal of Molecular Sciences. Research fields span autophagy, lysosomal/endosomal trafficking, cancer biology, viral infections, neurodegeneration, diabetic kidney disease, exosome biology, and cell death mechanisms.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Nlp promotes autophagy through facilitating the interaction of Rab7 and FYCO1. | ||
Cell Metab Oral GLP-1 receptor agonist promotes astrocyte-neuron lactate and lipid transfer with neuroprotective effects. | ||
Immunity Targeting intracellular oncoproteins with dimeric IgA promotes expulsion from the cytoplasm and immune-mediated control of epithelial cancers | ||
Nat Biomed Eng A deep learning and large language hybrid workflow for omics interpretation. | ||
Reviews
What customers say
Most reviewers rate this RAB7A antibody highly (average ~4.5/5), praising its performance in Western Blot, Immunofluorescence, and Immunohistochemistry. Users consistently report clean bands and strong specific signal at dilutions ranging from 1:50 to 1:2000. It works well in various cell lines including HEK293T, THP-1, NSC-34, and cancer cell lines. A couple of reviewers noted occasional weak signal or nonspecific high molecular weight bands in certain samples, but overall the antibody is considered reliable and reproducible.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Angélique (Verified Customer) (04-27-2026) | western-blot on mouse liver tumors compared to non-tumor
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Iram (Verified Customer) (03-23-2026) | It gives very good bands
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Sai Sindhura (Verified Customer) (01-08-2026) | RAB7A antibody is very good for IF
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Iram (Verified Customer) (09-18-2025) | very good antibody
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Christine (Verified Customer) (08-05-2025) | Detects endogenous Rab7a in total cell lysates of HEK293T. Clean, good antibody
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Sophy (Verified Customer) (03-07-2023) | The antibody worked well in western with 1:1000 dilution. The signal was somewhat weak but that could also be due to the cell types that were used.
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Malgorzata (Verified Customer) (08-17-2022) | The antibody worked on my positive control: Lysate of THP1 cell line. Nevertheless, for my samples it gave strong nonspecific signal in high MW
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Azita (Verified Customer) (06-17-2021) | Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by RAB7A Polyclonal antibody at dilution of 1:50 showed strong labelling.
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Laura (Verified Customer) (01-15-2020) | Works well for Western Blot at 1:1000 dilution.
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Aamir (Verified Customer) (01-08-2020) | WB at 1 in 500IF at 1 in 250
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Benjamin (Verified Customer) (12-10-2019) | Has worked well multiple times with dilution of 1:1000 with the RAB7A antibody. I always incubate at 4 degrees overnight in 3% milk TBST solution.
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