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  • KD/KO Validated

RAB7A Polyclonal antibody

RAB7A Polyclonal Antibody for WB, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (3)





Cat no : 55469-1-AP


PRO2706, RAB7, RAB7A, Ras related protein Rab 7a

Tested Applications

Positive WB detected inL-929 cells, NIH/3T3 cells, A431 cells, SH-SY5Y cells, RAW 264.7 cells, C6 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman kidney tissue, human skeletal muscle tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inA431 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55469-1-AP targets RAB7A in WB, IP, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey, Zebrafish, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name RAB7A, member RAS oncogene family
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberNM_004637
Gene Symbol RAB7A
Gene ID (NCBI) 7879
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB7A, also named as RAB7, belongs to the small GTPase superfamily and Rab family. It is involved in late endocytic transport. RAB7A contributes to the maturation of phagosomes (acidification). Defects in RAB7A are the cause of Charcot-Marie-Tooth disease type 2B (CMT2B). This antibody is specific to RAB7A.


Product Specific Protocols
WB protocol for RAB7A antibody 55469-1-APDownload protocol
IHC protocol for RAB7A antibody 55469-1-APDownload protocol
IP protocol for RAB7A antibody 55469-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Res

RAB31 marks and controls an ESCRT-independent exosome pathway.

Authors - Denghui Wei

Nat Commun

C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy

Authors - Shen Zhang


SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes

Authors - Wang Zheng

Nat Commun

Dstyk mutation leads to congenital scoliosis-like vertebral malformations in zebrafish via dysregulated mTORC1/TFEB pathway.

Authors - Xianding Sun

Nat Commun

CHML promotes liver cancer metastasis by facilitating Rab14 recycle.

Authors - Tian-Wei Chen


Bunyavirus SFTSV exploits autophagic flux for viral assembly and egress

Authors - Jia-min Yan


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Sophy (Verified Customer) (03-07-2023)

The antibody worked well in western with 1:1000 dilution. The signal was somewhat weak but that could also be due to the cell types that were used.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: NSCLC cells

Malgorzata (Verified Customer) (08-17-2022)

The antibody worked on my positive control: Lysate of THP1 cell line. Nevertheless, for my samples it gave strong nonspecific signal in high MW

  • Applications: Western Blot
  • Primary Antibody Dilution: 1000
  • Cell Tissue Type: THP-1
RAB7A Antibody Western Blot validation (1000 dilution) in THP-1 (Cat no:55469-1-AP)

Azita (Verified Customer) (06-17-2021)

Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by RAB7A Polyclonal antibody at dilution of 1:50 showed strong labelling.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: NSC-34 cells

Laura (Verified Customer) (01-15-2020)

Works well for Western Blot at 1:1000 dilution.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T

Aamir (Verified Customer) (01-08-2020)

WB at 1 in 500IF at 1 in 250

  • Applications: Western Blot, Immunohistochemistry,
  • Cell Tissue Type: HEKs

Benjamin (Verified Customer) (12-10-2019)

Has worked well multiple times with dilution of 1:1000 with the RAB7A antibody. I always incubate at 4 degrees overnight in 3% milk TBST solution.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293 Lysate
RAB7A Antibody Western Blot, validation (1:1000 dilution) in HEK293 Lysate (Cat no:55469-1-AP)