Anticorps Polyclonal de lapin anti-CIRBP

• Phare
• Validé par KD/KO

CIRBP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA

Conjugaison

Non conjugué

Publications

76

N° de cat : 10209-2-AP

Synonymes

CIRP, A18HNRNP, A18 hnRNP

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Galerie de données de validation

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  WB analysis of PC-12 using 10209-2-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 10209-2-AP (CIRBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 cells using 10209-2-AP

  WB result of CIRBP antibody (10209-2-AP, 1:500) with si-control and si-CIRBP transfected A549 cell.

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  WB analysis of HepG2 using 10209-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10209-2-AP (CIRBP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10209-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10209-2-AP (CIRBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 10209-2-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 10209-2-AP (CIRBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-13 using 10209-2-AP

  PC-13 cells were subjected to SDS PAGE followed by western blot with 10209-2-AP (CIRBP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10209-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10209-2-AP (CIRBP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 10209-2-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10209-2-AP (CIRBP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10209-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10209-2-AP (CIRBP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 10209-2-AP

  IP result of anti-CIRBP (IP:10209-2-AP, 3ug; Detection:10209-2-AP 1:500) with HepG2 cells lysate 6000ug.

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  IHC staining of human pancreas cancer using 10209-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10209-2-AP (CIRBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 10209-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10209-2-AP (CIRBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 10209-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10209-2-AP (CIRBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10209-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10209-2-AP (CIRBP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 10209-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10209-2-AP (CIRBP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 10209-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10209-2-AP (CIRBP antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 10209-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10209-2-AP (CIRBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10209-2-AP

  Immunofluorescent analysis of HepG2 cells using 10209-2-AP (CIRBP antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  FC experiment of A549 using 10209-2-AP

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human CIRBP (10209-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules PC-12, cellules A549, cellules HEK-293, cellules HepG2, cellules PC-13, cellules Y79, tissu cérébral de souris, tissu testiculaire de souris
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu de cancer du sein humain, tissu pancréatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2
• Résultats positifs en FC (Intra): cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 19 publications below
• WB: See 62 publications below
• IHC: See 13 publications below
• IF: See 11 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below
• RIP: See 2 publications below

Informations sur le produit

10209-2-AP cible CIRBP dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris, squirrel
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CIRBP Protéine recombinante Ag0280
• Nom complet: cold inducible RNA binding protein
• Masse moléculaire calculée: 172 aa, 19 kDa
• Poids moléculaire observé: 19 kDa
• Numéro d’acquisition GenBank: BC000403
• Symbole du gène: CIRBP
• Identification du gène (NCBI): 1153
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CIRBP, also named as A18HNRNP and CIRP, is a cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival[PMID: 19158277]. CIRBP is also involved in cap-independent translation upon moderate cold-shock. It acts as a translational activator. CIRBP is a suppressive rather stimulatory effect on proliferation[PMID: 19777567]. CIRBP can translocate from nucleus to cytoplasm under stresses such as UV irradation or heat shock. Suppression of CIRBP increased the apoptotic cell population of neural stem cells at moderate low temperature[PMID: 20735994]. This antibody (Catalog# 10209-2-AP) is a rabbit polyclonal antibody raised against the full-length CIRBP of human origin.

Publications

Species	Application	Title
mouse	WB	Nature; RBM3 mediates structural plasticity and protective effects of cooling in neurodegeneration.; Authors - Diego Peretti
human,squirrel	WB	Cell; iPSCs from a Hibernator Provide a Platform for Studying Cold Adaptation and Its Potential Medical Applications.; Authors - Jingxing Ou
rat	WB	Nat Med; Cold-inducible RNA-binding protein (CIRP) triggers inflammatory responses in hemorrhagic shock and sepsis.; Authors - Qiang Xiaoling X
human	WB	Mol Cell; Altered m6A Modification of Specific Cellular Transcripts Affects Flaviviridae Infection.; Authors - Nandan S Gokhale
human	WB	Nucleic Acids Res; Alternative splicing induced by bacterial pore-forming toxins sharpens CIRBP-mediated cell response to Listeria infection; Authors - Morgane Corre
human	WB	EBioMedicine; Hypothermic Preconditioning of Human Cortical Neurons Requires Proteostatic Priming.; Authors - Nina Marie Rzechorzek

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