RBM3 Polyclonal antibody

• Featured Product
• KD/KO Validated

RBM3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugate

Unconjugated

Publications

39

Cat no : 14363-1-AP

Synonyms

RNPL, RNA-binding protein 3, RNA-binding motif protein 3, RNA binding motif protein 3, Putative RNA binding protein 3

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Validation Data Gallery

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  WB analysis using 14363-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14363-1-AP (RBM3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14363-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14363-1-AP (RBM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 14363-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 14363-1-AP (RBM3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 14363-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14363-1-AP (RBM3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14363-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14363-1-AP (RBM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 14363-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14363-1-AP (RBM3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 14363-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14363-1-AP (RBM3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 14363-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14363-1-AP (RBM3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 14363-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14363-1-AP (RBM3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 14363-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14363-1-AP (RBM3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 14363-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14363-1-AP (RBM3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 14363-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14363-1-AP (RBM3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 14363-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14363-1-AP (RBM3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 14363-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14363-1-AP (RBM3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 14363-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14363-1-AP (RBM3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 14363-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM3 antibody (14363-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, A375 cells, Raji cells, HepG2 cells, Jurkat cells, NIH/3T3 cells
• Positive IHC detected in: human testis tissue, human lung tissue, human brain tissue, human kidney tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 31 publications below
• IHC: See 7 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• RIP: See 3 publications below

Product Information

14363-1-AP targets RBM3 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, squirrel
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RBM3 fusion protein Ag5934
• Full Name: RNA binding motif (RNP1, RRM) protein 3
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 17-20 kDa
• GenBank Accession Number: BC006825
• Gene Symbol: RBM3
• Gene ID (NCBI): 5935
• RRID: AB_2269266
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBM3, also named as RNPL, is a cold-inducible mRNA binding protein that enhances global protein synthesis at both physiological and mild hypothermic temperatures. It reduces the relative abundance of microRNAs, when overexpressed. RBM3 enhances phosphoryaltion of translation initiation factors and active polysome formation. It is up-regulated in human tumors. RBM3 is a potential proto-oncogenic proteins upon overexpression. (PMID: 19900510 ).

Publications

Species	Application	Title
mouse	WB	Nature; RBM3 mediates structural plasticity and protective effects of cooling in neurodegeneration.; Authors - Diego Peretti ; KD Validated
human,squirrel	WB	Cell; iPSCs from a Hibernator Provide a Platform for Studying Cold Adaptation and Its Potential Medical Applications.; Authors - Jingxing Ou
		EBioMedicine; The RNA-binding protein RBM3 promotes cell proliferation in hepatocellular carcinoma by regulating circular RNA SCD-circRNA 2 production.; Authors - Wei Dong
human	WB	Clin Transl Med; LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing; Authors - Qi Wen Chen
mouse	WB	J Cell Biol; Cold-induced protein RBM3 orchestrates neurogenesis via modulating Yap mRNA stability in cold stress.; Authors - Wenlong Xia ; KD Validated
mouse	WB	Front Cell Dev Biol; The RNA-Binding Protein RBM3 Promotes Neural Stem Cell (NSC) Proliferation Under Hypoxia.; Authors - Jingyi Yan

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