EIF4G1 Polyclonal antibody

• Featured Product
• KD/KO Validated

EIF4G1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

24

Cat no : 15704-1-AP

Synonyms

eIF-4G 1, EIF4G, EIF4F, eIF 4G1, eIF 4G 1

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Validation Data Gallery

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  WB analysis using 15704-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15704-1-AP (EIF4G1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15704-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15704-1-AP (EIF4G1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15704-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15704-1-AP (EIF4G1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 15704-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15704-1-AP (EIF4G1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15704-1-AP

  IP result of anti-EIF4G1 (IP:15704-1-AP, 5ug; Detection:15704-1-AP 1:500) with HeLa cells lysate 2000ug.

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  IHC staining of human breast cancer using 15704-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15704-1-AP (EIF4G1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15704-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15704-1-AP (EIF4G1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 15704-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF4G1 antibody (15704-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HepG2 using 15704-1-AP

  Immunofluorescent analysis of HepG2 cells using 15704-1-AP (EIF4G1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  IF Staining of HepG2 using 15704-1-AP

  Immunofluorescent analysis of Ethacrynic acid treated HepG2 cells using 15704-1-AP (EIF4G1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: BxPC-3 cells, HeLa cells, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, Ethacrynic acid treated HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 16 publications below
• IHC: See 4 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

15704-1-AP targets EIF4G1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EIF4G1 fusion protein Ag8342
• Full Name: eukaryotic translation initiation factor 4 gamma, 1
• Calculated Molecular Weight: 1600 aa, 176 kDa
• Observed Molecular Weight: 250 kDa
• GenBank Accession Number: BC007788
• Gene Symbol: EIF4G1
• Gene ID (NCBI): 1981
• RRID: AB_2261979
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Eukaryotic cellular messenger RNAs are posttranscriptionally modified by addition of an m(7)GTP moiety to the 5-prime terminus, referred to as a cap. Recognition of the cap structure and unwinding of mRNA secondary structure during the initiation phase of protein synthesis is catalyzed by initiation factors of the eIF4 group. EIF4G1, a subunit of eIF4 gamma, forms various complexes with the other eIF4 polypeptides [PMID: 7601469]. Mutations in the EIF4G1 gene, encoding a component of the eIF4F translation initiation complex, were recently reported as a possible cause for the autosomal dominant form of Parkinson's disease [PMID:22658323]. The calcualted molecular weight of EIF4G1 is 175 kDa, but modified EIF4G1 is about 220-240 kDa. (PMID: 18426977 )

Publications

Species	Application	Title
	CoIP	Mol Cell; The oncomicropeptide APPLE promotes hematopoietic malignancy by enhancing translation initiation.; Authors - Linyu Sun
human	IF	J Extracell Vesicles; Extracellular vesicles rich in HAX1 promote angiogenesis by modulating ITGB6 translation.; Authors - Bo You
human	WB	Proc Natl Acad Sci U S A; Cellular cap-binding protein, eIF4E, promotes picornavirus genome restructuring and translation.; Authors - Brian C Avanzino
human	WB	PLoS Genet; SAMHD1 Inhibits LINE-1 Retrotransposition by Promoting Stress Granule Formation.; Authors - Siqi Hu
mouse	WB,IF	Diabetes; Translational Factor eIF4G1 Regulates Glucose Homeostasis and Pancreatic β-Cell Function.; Authors - Seokwon Jo ; KO Validated
human	WB	Virol Sin; Baicalein suppresses Coxsackievirus B3 replication by inhibiting caspase-1 and viral protease 2A; Authors - Yanyan Dong