Anticorps Polyclonal de lapin anti-CHMP2A

• Phare
• Validé par KD/KO

CHMP2A Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

45

Avis (1)

N° de cat : 10477-1-AP

Synonymes

CHMP2, CHMP2A, chromatin modifying protein 2A, hVps2 1, VPS2, Vps2 1, VPS2A

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Galerie de données de validation

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  WB analysis of HepG2 using 10477-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10477-1-AP (CHMP2A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10477-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10477-1-AP (CHMP2A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10477-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10477-1-AP (CHMP2A Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 10477-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10477-1-AP (CHMP2A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10477-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10477-1-AP (CHMP2A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HEK-293, cellules HeLa
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 16 publications below
• WB: See 27 publications below
• IF: See 22 publications below
• IP: See 1 publications below

Informations sur le produit

10477-1-AP cible CHMP2A dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CHMP2A Protéine recombinante Ag0719
• Nom complet: chromatin modifying protein 2A
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC002502
• Symbole du gène: CHMP2A
• Identification du gène (NCBI): 27243
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CHMP2A, also known as Vps2-1, belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. CHMP2A is a component of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in the degradation of surface receptor proteins and the formation of endocytic multivesicular bodies (MVBs). It has been shown that CHMP2A can interact with and regulate the function of the AAA-ATPase VPS4B (PMID: 15173323). CHMP2A is also reported to be involved in HIV budding (PMID: 21396898; 23051622).

Publications

Species	Application	Title
human	IF	Nature; Nuclear envelope assembly defects link mitotic errors to chromothripsis.; Authors - Shiwei Liu
human	WB	Cell; Mammalian hybrid pre-autophagosomal structure HyPAS generates autophagosomes; Authors - Suresh Kumar ; KD Validated
human	WB, IF	Nature; ESCRT-III controls nuclear envelope reformation.; Authors - Yolanda Olmos ; KD Validated
human	WB,IF	Nature; Spastin and ESCRT-III coordinate mitotic spindle disassembly and nuclear envelope sealing.; Authors - Marina Vietri ; KD Validated
human	WB,IF	Nat Cell Biol; Unrestrained ESCRT-III drives micronuclear catastrophe and chromosome fragmentation.; Authors - Marina Vietri ; KD Validated
human	IF	Nat Cell Biol; Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.; Authors - Vincent Mercier

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Stephen (Verified Customer) (04-08-2019)

IF image of Methanol fixed a dividing HeLa cell Stained with CHMP2A antibody (green) and Tubulin Antibody (red)

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: HeLa

CHMP2A Antibody Immunofluorescence, validation (1:300 dilution) in HeLa (Cat no:10477-1-AP)

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