CHMP1B Polyclonal antibody
CHMP1B Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 14639-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, human heart tissue, mouse heart tissue, rat heart tissue, HeLa cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 4 publications below |
IF | See 7 publications below |
Product Information
14639-1-AP targets CHMP1B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CHMP1B fusion protein Ag6222 |
Full Name | chromatin modifying protein 1B |
Calculated Molecular Weight | 22 kDa |
Observed Molecular Weight | 28 kDa |
GenBank Accession Number | BC065933 |
Gene Symbol | CHMP1B |
Gene ID (NCBI) | 57132 |
RRID | AB_2079353 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for CHMP1B antibody 14639-1-AP | Download protocol |
IHC protocol for CHMP1B antibody 14639-1-AP | Download protocol |
IP protocol for CHMP1B antibody 14639-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Cell Biol Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation. | ||
J Extracell Vesicles Accessory ESCRT-III proteins are conserved and selective regulators of Rab11a-exosome formation | ||
Dev Cell NPC1-mTORC1 Signaling Couples Cholesterol Sensing to Organelle Homeostasis and Is a Targetable Pathway in Niemann-Pick Type C. | ||
Elife (Cambridge) Structure of cellular ESCRT-III spirals and their relationship to HIV budding. | ||
MBio Mycobacterium tuberculosis Type VII Secretion System Effectors Differentially Impact the ESCRT Endomembrane Damage Response. | ||
Life Sci Alliance Vaccinia virus hijacks ESCRT-mediated multivesicular body formation for virus egress. |