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CHMP1A Polyclonal antibody
CHMP1A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 15761-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, mouse lung tissue, HeLa cells, A431 cells, mouse kidney tissue |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | mouse brain tissue, human lung tissue, mouse testis tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 3 publications below |
WB | See 6 publications below |
IHC | See 1 publications below |
IF | See 3 publications below |
Product Information
15761-1-AP targets CHMP1A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CHMP1A fusion protein Ag8389 |
Full Name | chromatin modifying protein 1A |
Calculated Molecular Weight | 196 aa, 22 kDa |
Observed Molecular Weight | 33 kDa, 25 kDa |
GenBank Accession Number | BC007527 |
Gene Symbol | CHMP1A |
Gene ID (NCBI) | 5119 |
RRID | AB_2229399 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Charged multivesicular body protein 1A (CHMP1A), also known as chromatin modifying protein 1A, is a member of the ESCRT-III (endosomal sorting complex required for transport III) complex which is involved in degradation of internalized transmembrane receptor proteins and formation of multivesicular bodies (MVBs) (PMID: 16730941). CHMP1A has both nuclear and cytoplasmic distributions (PMID: 11559748; 11559747). Subcellular localization of CHMP1A seems to vary depending on the cell type (PMID: 23023333). CHMP1A is required for MVBs formation and has a role in stable gene silencing within the nucleus. Besides, the tumor suppressor role of CHMP1A has also been reported (PMID: 18787405; 22261332).
Protocols
Product Specific Protocols | |
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WB protocol for CHMP1A antibody 15761-1-AP | Download protocol |
IHC protocol for CHMP1A antibody 15761-1-AP | Download protocol |
IF protocol for CHMP1A antibody 15761-1-AP | Download protocol |
IP protocol for CHMP1A antibody 15761-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Cell Biol Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation. | ||
J Extracell Vesicles Accessory ESCRT-III proteins are conserved and selective regulators of Rab11a-exosome formation | ||
Nat Commun A single genetic locus controls both expression of DPEP1/CHMP1A and kidney disease development via ferroptosis.
| ||
Nat Commun Mechanism of Ca²⁺-triggered ESCRT assembly and regulation of cell membrane repair. | ||
Cell Rep The ESCRT-III Protein CHMP1A Mediates Secretion of Sonic Hedgehog on a Distinctive Subtype of Extracellular Vesicles.
| ||
Cell Rep Resolving ESCRT-III Spirals at the Intercellular Bridge of Dividing Cells Using 3D STORM.
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